Incorporation of impurity to a tetragonal lysozyme crystal

Kazuo Kurihara, Satoru Miyashita, Gen Sazaki, Toshitaka Nakada, Stephen D. Durbin, Hiroshi Komatsu, Tetsuhiko Ohba, Kazuo Ohki

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Concentration of a phosphor-labeled impurity (ovalbumin) incorporated into protein (hen egg white lysozyme) crystals during growth was measured by fluorescence.This technique enabled us to measure the local impurity concentration in a crystal quantitatively. Impurity concentration increased with growth rate, which could not be explained by two conventional models (equilibrium adsorption model and Burton-Prim-Slichter model); a modified model is proposed. Impurity concentration also increased with the pH of the solution. This result is discussed considering the electrostatic interaction between the impurity and the crystallizing species.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalJournal of Crystal Growth
Volume196
Issue number2-4
DOIs
Publication statusPublished - 1999 Jan 15

Keywords

  • Crystal growth
  • Fluorescence
  • Impurity
  • Incorporation
  • Lysozyme
  • Ovalbumin

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

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