Incorporation of capillary-like structures into dermal cell sheets constructed by magnetic force-based tissue engineering

Kosuke Ino, Akira Ito, Hirohito Kumazawa, Hideaki Kagami, Minoru Ueda, Hiroyuki Honda

Research output: Contribution to journalArticle

23 Citations (Scopus)


One of the major allenges in tissue engineering remains the construction of vascularized 3D transplants in vitro. We recently proposed novel technologies, termed "magnetic force-based tissue engineering" (Mag-TE), to establish three-dimensional (3D) tissues without using scaffolds. Magnetite cationic liposomes (MCLs), which contain 10-nm magnetite nanoparticles in order to improve accumulation of magnetite nanoparticles in target cells, were used to magnetically label normal human dermal fibroblasts (NHDFs). Magnetically labeled NHDFs were seeded onto ultralow-attachment plates. When a magnet was placed under the plate, cells accumulate on the bottom of the well. After a 24-h-incubation period, the cells form a sheet-like structure, which contains the major dermal extracellular matrix (ECM) components (fibronectin and type I collagen) within the NHDF sheet. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NHDF sheets by two methods: HUVECs and NHDFs were mixed and then allowed to form cell sheets by Mag-TE; or NEW sheets were constructed by Mag-TE and HUVECs were subsequently seeded onto NEW sheets. These methods gave tube-like formation of HAECs, resembling early capillaries, within or on the surface NHDF sheets after short-term 3D co-culture, thus suggesting that Mag-TE may be useful for constructing 3D-tissue involving capillaries.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
Issue number1
Publication statusPublished - 2007 Jan 19
Externally publishedYes


  • Angiogenesis
  • Co-culture
  • Dermis
  • Liposome
  • Magnetite nanoparticle

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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