Incorporation of an inducible nucleotide analog into DNA by DNA polymerases

Md Monsur Ali, Shuhei Imoto, Yingfu Li, Shigeki Sasaki, Fumi Nagatsugi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5′-triphosphate of 2′-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.

Original languageEnglish
Pages (from-to)2859-2863
Number of pages5
JournalBioorganic and Medicinal Chemistry
Volume17
Issue number7
DOIs
Publication statusPublished - 2009 Apr 1

Keywords

  • 2-Amino-6-vinylpurine (AVP)
  • 5′-Triphosphate
  • DNA polymerase
  • Inducible alkylating system
  • Modified oligonucleotides
  • Post-synthetic conjugation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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