Inbreeding effect towards genetic markers and thermal tolerance in guppy Poecilia reticulate

Aim: The guppy was used to demonstrate the effect of continues full-sib mating to the genetic changes and occurrence of inbreeding depression through heat stress tolerance. Methodology: Inbreeding depression were assessed from data collected from microsatellite markers and thermal tolerance experiment. The AY and NA1 strains were selected for this experiment. Results: With the progress in the inbred generations, significant decrease in thermal tolerance was observed. The observed (Ho) and expected (He) heterozygosity also decreased, indicating the on going inbred process. Significant correlation was observed only in the NA1 strain between thermal tolerances with multilocus heterozygosity (MLH) experiment both in Internal Relatedness (IR) and Standardize Heterozygosity (SH), respectively. Interpretation: MLH can be used to determent inbreeding depression only when the samples are severely inbred.