Inactivation of sonic hedgehog signaling and polydactyly in limbs of Hereditary Multiple Malformation, a novel type of talpid mutant

Yoshiyuki Matsubara, Mikiharu Nakano, Kazuki Kawamura, Masaoki Tsudzuki, Jun Ichi Funahashi, Kiyokazu Agata, Yoichi Matsuda, Atsushi Kuroiwa, Takayuki Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hereditary Multiple Malformation (HMM) is a naturally occurring, autosomal recessive, homozygous lethal mutation found in Japanese quail. Homozygote embryos (hmm -/- ) show polydactyly similar to talpid2 and talpid3 mutants. Here we characterize the molecular profile of the hmm -/- limb bud and identify the cellular mechanisms that cause its polydactyly. The hmm -/- hmm -/- limb bud shows a severe lack of sonic hedgehog (SHH) signaling, and the autopod has 4 to 11 unidentifiable digits with syn-, poly-, and brachydactyly. The Zone of Polarizing Activity (ZPA) of the hmm -/- limb bud does not show polarizing activity regardless of the presence of SHH protein, indicating that either the secretion pathway of SHH is defective or the SHH protein is dysfunctional. Furthermore, mesenchymal cells in the hmm -/- limb bud do not respond to ZPA transplanted from the normal limb bud, suggesting that signal transduction downstream of SHH is also defective. Since primary cilia are present in the hmm -/- limb bud, the causal gene must be different from talpid2 and talpid3. In the hmm -/- limb bud, a high amount of GLI3A protein is expressed and GLI3 protein is localized to the nucleus. Our results suggest that the regulatory mechanism of GLI3 is disorganized in the hmm -/- limb bud.

Original languageEnglish
Article number149
JournalFrontiers in Cell and Developmental Biology
Volume4
Issue numberDEC
DOIs
Publication statusPublished - 2016 Dec 27

Keywords

  • Hereditary Multiple Malformation
  • Polydactyly
  • Quail
  • Sonic hedgehog

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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