Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion

Marcos Galasso, Jordan J. Feld, Yui Watanabe, Mauricio Pipkin, Cara Summers, Aadil Ali, Robert Qaqish, Manyin Chen, Rafaela V.P. Ribeiro, Khaled Ramadan, Layla Pires, Vanderlei S. Bagnato, Cristina Kurachi, Vera Cherepanov, Gray Moonen, Anajara Gazzalle, Thomas K. Waddell, Mingyao Liu, Shaf Keshavjee, Brian C. WilsonAtul Humar, Marcelo Cypel

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Availability of organs is a limiting factor for lung transplantation, leading to substantial mortality rates on the wait list. Use of organs from donors with transmissible viral infections, such as hepatitis C virus (HCV), would increase organ donation, but these organs are generally not offered for transplantation due to a high risk of transmission. Here, we develop a method for treatment of HCV-infected human donor lungs that prevents HCV transmission. Physical viral clearance in combination with germicidal light-based therapies during normothermic ex-vivo Lung Perfusion (EVLP), a method for assessment and treatment of injured donor lungs, inactivates HCV virus in a short period of time. Such treatment is shown to be safe using a large animal EVLP-to-lung transplantation model. This strategy of treating viral infection in a donor organ during preservation could significantly increase the availability of organs for transplantation and encourages further clinical development.

Original languageEnglish
Article number481
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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