In vivo optical recording of brain interneuron activities from a drosophila male on a treadmill

Soh Kohatsu, Masayuki Koganezawa, Daisuke Yamamoto

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Recent development of genetically encoded calcium indicators (GECIs) allows us to directly monitor neural activities in in vivo preparations from various organisms, enabling the exploration of neural substrates for complex behaviors. As a showcase for such renovated neuroethology with GECIs, we describe a novel method to record optically interneuron activities from the brain of a tethered male fruit fly Drosophila melanogaster when contact chemosensory stimulation is given to his foreleg. In this protocol, the recording is made on the preparation in which a fly is put on an air-supported styrofoam ball, keeping the moving fly in position to allow monitoring of the activity of central neurons from the fly whose movement is minimally restricted. This protocol is applicable to the analysis of other behaviors induced by stimuli of different modalities with small modifications.

    Original languageEnglish
    Title of host publicationGenetically Encoded Functional Indicators
    PublisherHumana Press Inc.
    Pages103-112
    Number of pages10
    ISBN (Print)9781627030137
    DOIs
    Publication statusPublished - 2012

    Publication series

    NameNeuromethods
    Volume72
    ISSN (Print)0893-2336
    ISSN (Electronic)1940-6045

    Keywords

    • Calcium imaging
    • Courtship
    • Drosophila
    • Fruitless
    • Yellow Cameleon

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology, Toxicology and Pharmaceutics(all)
    • Psychiatry and Mental health

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