In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe

Yoshifumi Yamaguchi, Erina Kuranaga, Yu Ichiro Nakajima, Akiko Koto, Kiwamu Takemoto, Masayuki Miura

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Caspases, which constitute a family of cysteine proteases, are highly conserved in multicellular organisms and function as a central player in apoptosis. The detection of apoptosis is intrinsically difficult because dying cells are rapidly removed from tissues by phagocytosis. Thus, the development of a method for detecting caspase activation is critical for the in vivo study of apoptosis. In this chapter, we describe a genetically encoded fluorescent probe for live imaging of caspase activation.

Original languageEnglish
Title of host publicationRegulated Cell Death Part A
Subtitle of host publicationApoptotic Mechanisms
PublisherAcademic Press Inc.
Pages299-325
Number of pages27
ISBN (Print)9780124171589
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume544
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Apoptosis
  • FRET probe
  • caspase
  • drosophila
  • live-imaging
  • mouse

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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