In vivo molecular imaging analysis of a Nasal vaccine that induces protective immunity against botulism in nonhuman primates

Yoshikazu Yuki, Tomonori Nochi, Norihiro Harada, Yuko Katakai, Hiroaki Shibata, Mio Mejima, Tomoko Kohda, Daisuke Tokuhara, Shiho Kurokawa, Yuko Takahashi, Fumiko Ono, Shunji Kozaki, Keiji Terao, Hideo Tsukada, Hiroshi Kiyono

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Nasal administrationis an effective route foraneedle-free vaccine. However, nasally administered Ags have the potential toreach the CNS directly from the nasal cavity, thus raising safety concerns.Inthis study, weperformed real-time quantitative trackingofanasal vaccine candidate for botulism, which is a nontoxic subunit fragment of Clostridium botulinum type A neurotoxin (BoHc/A) effective in the induction of the toxin-neutralizing immune response, by using18F-labeled BoHc/A-positron-emission tomography, an in vivo molecular imaging method. This method provides results that are consistent with direct counting of [18F] radioactivity or the traditional [ 111In]-radiolabel method in dissected tissues of mice and nonhuman primates. We found no deposition of BoHc/A in the cerebrum or olfactory bulb after nasal administration of18F-labeled BoHc/A in both animals. We also established a real-time quantitative profile of elimination of this nasal vaccine candidate and demonstrated that it induces highly protective immunity against botulism in nonhuman primates. Our findings demonstrate the efficiency and safety of a nasal vaccine candidate against botulism in mice and nonhuman primates using in vivo molecular imaging.

Original languageEnglish
Pages (from-to)5436-5443
Number of pages8
JournalJournal of Immunology
Volume185
Issue number9
DOIs
Publication statusPublished - 2010 Nov 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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