In vivo interaction between the human dehydrodolichyl diphosphate synthase and the Niemann-Pick C2 protein revealed by a yeast two-hybrid system

Yugesh Kharel, Seiji Takahashi, Satoshi Yamashita, Tanetoshi Koyama

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Dehydrodolichyl diphosphate (DedolPP) synthase catalyzes the sequential condensation of isopentenyl diphosphate with farnesyl diphosphate to synthesize DedolPP, a biosynthetic precursor for dolichol which plays an important role as a sugar-carrier lipid in the biosynthesis of glycoprotein in eukaryotic cells. During certain pathological processes like Alzheimer's disease or some neurological disorders, dolichol has been shown to accumulate in human brain. In order to understand the regulatory mechanism of dolichol in eukaryotes, we performed a yeast two-hybrid screen using full length human DedolPP synthase gene [Endo et al. BBA 1625 (2003) 291] as a bait to find some proteins specifically interacting with the enzyme. We identified Niemann-Pick Type C2 protein (NPC2) to show a specific interaction with human DedolPP synthase. This interaction was further confirmed by in vitro co-immunoprecipitation experiment, indicating the possible physiological interaction between NPC2 and DedolPP synthase proteins in human.

Original languageEnglish
Pages (from-to)198-203
Number of pages6
JournalBiochemical and biophysical research communications
Volume318
Issue number1
DOIs
Publication statusPublished - 2004 May 21

Keywords

  • Dehydrodolichyl diphosphate synthase
  • NPC2
  • Niemann-Pick type C2 disease
  • Yeast two-hybrid system

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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