In vivo induction of necrosis in mice fibrosarcoma via intravenous injection of type B staphylococcal enterotoxin

Abbas Ali Imani Fooladi, Morteza Sattari, Zuhair Mohammad Hassan, Mehdi Mahdavi, Taghi Azizi, Akira Horii

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The bacterial superantigen staphylococcal enterotoxin B (SEB) is a potent inducer of cytotoxic T-cell activity and cytokine production in vivo. We investigated the possibility of the therapeutic application of SEB in patients with fibrosarcoma. The anti-tumor effect of SEB in mice with inoculated fibrosarcoma (WEHI-164) was examined by intravenous (IV) and intratumoral (IT) injection and the sizes of the inoculated tumors, IFN-γ production, and CD4+/CD8+ T cell infiltration were determined. The inoculated tumors were also examined histologically. In the mice in the IV-injected group, a significant reduction (P < 0.02) of tumor size was observed in comparison with mice in the IT-injected and control groups. Furthermore, the mice in the IV-injected group showed significantly higher levels of IFN-γ (P < 0.009) and CD4+/CD8+ T cell infiltration when compared with the other groups (P < 0.02). A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV-injected group (P < 0.05). Our present findings suggest that tumor cell death is caused by increased cytotoxic T-cell activity and cytokine levels in response to the IV injection of SEB and that SEB may be a good option for use as a novel therapy in patients with fibrosarcoma.

Original languageEnglish
Pages (from-to)2053-2059
Number of pages7
JournalBiotechnology Letters
Volume30
Issue number12
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • Anti-tumor effect
  • Cytokine
  • Mouse fibrosarcoma
  • Staphylococcal enterotoxin B (SEB)
  • WEHI-164

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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