In vivo induction of apoptosis and immune responses in mice by administration of lipopolysaccharide from Porphyromonas gingivalis

Emiko Isogai, Hiroshi Isogai, Koichi Kimura, Nobuhiro Fujii, Seiichi Takagi, Kimiharu Hirose, Masanobu Hayashi

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

In vivo administration of Porphyromonas gingivalis lipopolysaccharide (Pg- LPS) to mice induced apoptosis before a specific immune response. Apoptosis was associated with the expression of immunoglobulin and Ia on B cells and of CD5 and several markers on T cells. Apoptosis peaked in the spleen and lymph nodes on day 2, and the second peak occurred in the thymus on day 9. Tumor necrosis factor alpha (TNF-α) could mediate apoptosis, because the serum TNF-α levels were significantly higher than those of controls at 1 day before apoptosis and recombinant murine TNF-α induced apoptosis. The apoptosis induced by Pg-LPS was similar to that induced by Escherichia coli LPS in its basic manner, but it was unique in the response of thymus T cells. It was suggested that Pg-LPS could induce apoptosis for the elimination of early nonspecific activated lymphocytes.

Original languageEnglish
Pages (from-to)1461-1466
Number of pages6
JournalInfection and immunity
Volume64
Issue number4
DOIs
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'In vivo induction of apoptosis and immune responses in mice by administration of lipopolysaccharide from Porphyromonas gingivalis'. Together they form a unique fingerprint.

Cite this