TY - JOUR
T1 - In vivo detection of copper ions by magnetic resonance imaging using a prion-based contrast agent
AU - Makino, Satoshi
AU - Umemoto, Tomohiro
AU - Yamada, Hiroshi
AU - Yezdimer, Eric M.
AU - Tooyama, Ikuo
N1 - Funding Information:
Acknowledgments This work was supported by the Grant-in-Aid for Research and Development of an Intellectual Infrastructure Project of the New Energy and Industrial Technology Development Organization (NEDO) of Japan. The authors wish to sincerely thank Jeffrey P. Norenberg and Tamara Anderson at the University of New Mexico, College of Pharmacy, for running the SPECT experiments and Jack Hoppin, Jacob Hesterman, and Mary Germino at inviCRO, LLC (Boston, MA, USA) for help with the SPECT study design and image processing. The authors would also like to thank Takako Sasamura for help with the HPLC analysis and animal experiments.
PY - 2012/10
Y1 - 2012/10
N2 - Abnormal distributions of transition metals inside the body are potential diagnostic markers for several diseases, including Alzheimer's disease, Parkinson's disease, Wilson's disease, and cancer. In this article, we demonstrate that P57/Gd, a novel prion-based contrast agent, can selectively image tissues with excessive copper accumulation using magnetic resonance imaging (MRI). P57/Gd selectivity binds copper(II) over other physiologically relevant cations such as zinc, iron, manganese, and calcium. To simulate a metabolic copper disorder, we treated mice with an intraperitoneal injection of a CuSO4 solution to induce a renal copper overload. The MRI signal intensities from the renal cortex and medulla of copper spiked animals that were administered P57/Gd were found to correlate with the ex vivo copper concentrations determined by inductively coupled plasma mass spectrometry.
AB - Abnormal distributions of transition metals inside the body are potential diagnostic markers for several diseases, including Alzheimer's disease, Parkinson's disease, Wilson's disease, and cancer. In this article, we demonstrate that P57/Gd, a novel prion-based contrast agent, can selectively image tissues with excessive copper accumulation using magnetic resonance imaging (MRI). P57/Gd selectivity binds copper(II) over other physiologically relevant cations such as zinc, iron, manganese, and calcium. To simulate a metabolic copper disorder, we treated mice with an intraperitoneal injection of a CuSO4 solution to induce a renal copper overload. The MRI signal intensities from the renal cortex and medulla of copper spiked animals that were administered P57/Gd were found to correlate with the ex vivo copper concentrations determined by inductively coupled plasma mass spectrometry.
KW - Angiogenesis
KW - Copper
KW - In vivo test
KW - MRI (magnetic resonance imaging)
KW - Peptide
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U2 - 10.1007/s12010-012-9792-7
DO - 10.1007/s12010-012-9792-7
M3 - Article
C2 - 22810201
AN - SCOPUS:84869139867
SN - 0273-2289
VL - 168
SP - 504
EP - 518
JO - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
JF - Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
IS - 3
ER -
