TY - JOUR
T1 - In vivo crystallography at X-ray freeelectron lasers
T2 - The next generation of structural biology?
AU - Gallat, François Xavier
AU - Matsugaki, Naohiro
AU - Coussens, Nathan P.
AU - Yagi, Koichiro J.
AU - Boudes, Marion
AU - Higashi, Tetsuya
AU - Tsuji, Daisuke
AU - Tatano, Yutaka
AU - Suzuki, Mamoru
AU - Mizohata, Eiichi
AU - Tono, Kensuke
AU - Joti, Yasumasa
AU - Kameshima, Takashi
AU - Park, Jaehyun
AU - Song, Changyong
AU - Hatsui, Takaki
AU - Yabashi, Makina
AU - Nango, Eriko
AU - Itoh, Kohji
AU - Coulibaly, Fasséli
AU - Tobe, Stephen
AU - Ramaswamy, S.
AU - Stay, Barbara
AU - Iwata, So
AU - Chavas, Leonard M.G.
PY - 2014/7/17
Y1 - 2014/7/17
N2 - The serendipitous discoveryof the spontaneous growth of protein crystals inside cells has opened the field of crystallography to chemically unmodified samples directly available from their natural environment. On the one hand, through in vivo crystallography, protocols for protein crystal preparation can be highly simplified, although the technique suffers from difficulties in sampling, particularly in the extraction of the crystals fromthe cells partly due to their small sizes. On the other hand, the extremely intense X-ray pulses emerging fromX-ray freeelectron laser (XFEL) sources, along with the appearance of serial femtosecond crystallography (SFX) is a milestone for radiation damage-free protein structural studies but requires micrometre-size crystals. The combination of SFX with in vivo crystallography has the potential to boost the applicability of these techniques, eventually bringing the field to the point where in vitro sample manipulations will no longer be required, and direct imaging of the crystals from within the cells will be achievable. To fully appreciate the diverse aspects of sample characterization, handling and analysis, SFX experiments at the Japanese SPring-8 angstromcompact free-electron laserwere scheduled on various types of in vivo grown crystals. The first experiments have demonstrated the feasibility of the approach and suggest that future in vivo crystallography applications at XFELs will be another alternative to nano-crystallography.
AB - The serendipitous discoveryof the spontaneous growth of protein crystals inside cells has opened the field of crystallography to chemically unmodified samples directly available from their natural environment. On the one hand, through in vivo crystallography, protocols for protein crystal preparation can be highly simplified, although the technique suffers from difficulties in sampling, particularly in the extraction of the crystals fromthe cells partly due to their small sizes. On the other hand, the extremely intense X-ray pulses emerging fromX-ray freeelectron laser (XFEL) sources, along with the appearance of serial femtosecond crystallography (SFX) is a milestone for radiation damage-free protein structural studies but requires micrometre-size crystals. The combination of SFX with in vivo crystallography has the potential to boost the applicability of these techniques, eventually bringing the field to the point where in vitro sample manipulations will no longer be required, and direct imaging of the crystals from within the cells will be achievable. To fully appreciate the diverse aspects of sample characterization, handling and analysis, SFX experiments at the Japanese SPring-8 angstromcompact free-electron laserwere scheduled on various types of in vivo grown crystals. The first experiments have demonstrated the feasibility of the approach and suggest that future in vivo crystallography applications at XFELs will be another alternative to nano-crystallography.
KW - In vivo crystallography
KW - Serial femtosecond crystallography
KW - X-ray free-electron laser
UR - http://www.scopus.com/inward/record.url?scp=84902668991&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902668991&partnerID=8YFLogxK
U2 - 10.1098/rstb.2013.0497
DO - 10.1098/rstb.2013.0497
M3 - Article
C2 - 24914164
AN - SCOPUS:84902668991
VL - 369
JO - Philosophical Transactions of the Royal Society B: Biological Sciences
JF - Philosophical Transactions of the Royal Society B: Biological Sciences
SN - 0962-8436
IS - 1647
M1 - 20130497
ER -