In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu

Michiko Takahashi, Tomoko Nozoye, Nobuyuki Kitajima, Naoki Fukuda, Akiko Hokura, Yasuko Terada, Izumi Nakai, Yasuhiro Ishimaru, Takanori Kobayashi, Hiromi Nakanishi, Naoko K. Nishizawa

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

To investigate the flow of the metal nutrients iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) during rice seed germination, we performed microarray analysis to examine the expression of genes involved in metal transport. Many kinds of metal transporter genes were strongly expressed and their expression levels changed during rice seed germination. We found that metal transporter genes such as ZIP family has tendency to decrease in their expressions during seed germination. Furthermore, imaging of the distribution of elements (Fe, Mn, Zn, and Cu) was carried out using Synchrotron-based X-ray microfluorescence at the Super Photon ring-8 GeV (SPring-8) facility. The change in the distribution of each element in the seeds following germination was observed by in vivo monitoring. Iron, Mn, Zn, and Cu accumulated in the endosperm and embryos of rice seeds, and their distribution changed during rice seed germination. The change in the patterns of mineral localization during germination was different among the elements observed.

Original languageEnglish
Pages (from-to)39-51
Number of pages13
JournalPlant and Soil
Volume325
Issue number1
DOIs
Publication statusPublished - 2009 Jan
Externally publishedYes

Keywords

  • Germination
  • Metal nutrients
  • Rice seed
  • Synchrotron-based X-ray microfluorescence
  • Transporter

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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