In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent

Shigeo Matsuyama; Naoki Hamada; Keizo Ishii; Yuichiro Nozawa; Satoru Ohkura; Atsuki Terakawa; Yoshinobu Hatori; Kota Fujiki; Mitsuhiro Fujiwara; Sho Toyama

doi:10.1016/j.nimb.2014.11.039

In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent

Shigeo Matsuyama, Naoki Hamada, Keizo Ishii, Yuichiro Nozawa, Satoru Ohkura, Atsuki Terakawa, Yoshinobu Hatori, Kota Fujiki, Mitsuhiro Fujiwara, Sho Toyama

ENG - Quantum Science and Energy Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.

Original language	English
Pages (from-to)	123-126
Number of pages	4
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	348
DOIs	https://doi.org/10.1016/j.nimb.2014.11.039
Publication status	Published - 2015

Keywords

3D PIXE-micron-CT
Abdominal image
Contrast agent
Living Drosophila

ASJC Scopus subject areas

Nuclear and High Energy Physics
Instrumentation

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Matsuyama, S., Hamada, N., Ishii, K., Nozawa, Y., Ohkura, S., Terakawa, A., Hatori, Y., Fujiki, K., Fujiwara, M., & Toyama, S. (2015). In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 348, 123-126. https://doi.org/10.1016/j.nimb.2014.11.039

In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent. / Matsuyama, Shigeo; Hamada, Naoki; Ishii, Keizo; Nozawa, Yuichiro; Ohkura, Satoru; Terakawa, Atsuki; Hatori, Yoshinobu; Fujiki, Kota; Fujiwara, Mitsuhiro; Toyama, Sho.

In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 348, 2015, p. 123-126.

Research output: Contribution to journal › Article › peer-review

Matsuyama, S, Hamada, N, Ishii, K, Nozawa, Y, Ohkura, S, Terakawa, A, Hatori, Y, Fujiki, K, Fujiwara, M & Toyama, S 2015, 'In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 348, pp. 123-126. https://doi.org/10.1016/j.nimb.2014.11.039

Matsuyama, Shigeo ; Hamada, Naoki ; Ishii, Keizo ; Nozawa, Yuichiro ; Ohkura, Satoru ; Terakawa, Atsuki ; Hatori, Yoshinobu ; Fujiki, Kota ; Fujiwara, Mitsuhiro ; Toyama, Sho. / In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent. In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2015 ; Vol. 348. pp. 123-126.

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title = "In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent",

abstract = "In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.",

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note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research ((A) No. 23240077 ) from the Ministry of Education, Culture, Sports, Science, and Technology in Japan (MEXT) and a Grant-in-Aid for Challenging Exploratory Research grant (Grant No. 26630477 ) from the Japan Society for the Promotion of Science (JSPS).",

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AU - Matsuyama, Shigeo

AU - Hamada, Naoki

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AU - Nozawa, Yuichiro

AU - Ohkura, Satoru

AU - Terakawa, Atsuki

AU - Hatori, Yoshinobu

AU - Fujiki, Kota

AU - Fujiwara, Mitsuhiro

AU - Toyama, Sho

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research ((A) No. 23240077 ) from the Ministry of Education, Culture, Sports, Science, and Technology in Japan (MEXT) and a Grant-in-Aid for Challenging Exploratory Research grant (Grant No. 26630477 ) from the Japan Society for the Promotion of Science (JSPS).

PY - 2015

Y1 - 2015

N2 - In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.

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