TY - JOUR
T1 - In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent
AU - Matsuyama, Shigeo
AU - Hamada, Naoki
AU - Ishii, Keizo
AU - Nozawa, Yuichiro
AU - Ohkura, Satoru
AU - Terakawa, Atsuki
AU - Hatori, Yoshinobu
AU - Fujiki, Kota
AU - Fujiwara, Mitsuhiro
AU - Toyama, Sho
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research ((A) No. 23240077 ) from the Ministry of Education, Culture, Sports, Science, and Technology in Japan (MEXT) and a Grant-in-Aid for Challenging Exploratory Research grant (Grant No. 26630477 ) from the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
PY - 2015
Y1 - 2015
N2 - In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.
AB - In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.
KW - 3D PIXE-micron-CT
KW - Abdominal image
KW - Contrast agent
KW - Living Drosophila
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U2 - 10.1016/j.nimb.2014.11.039
DO - 10.1016/j.nimb.2014.11.039
M3 - Article
AN - SCOPUS:85027930657
VL - 348
SP - 123
EP - 126
JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
SN - 0168-583X
ER -