In vivo 3D PIXE-micron-CT imaging of Drosophila melanogaster using a contrast agent

Shigeo Matsuyama, Naoki Hamada, Keizo Ishii, Yuichiro Nozawa, Satoru Ohkura, Atsuki Terakawa, Yoshinobu Hatori, Kota Fujiki, Mitsuhiro Fujiwara, Sho Toyama

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In this study, we developed a three-dimensional (3D) computed tomography (CT) in vivo imaging system for imaging small insects with micrometer resolution. The 3D CT imaging system, referred to as 3D PIXE-micron-CT (PIXEμCT), uses characteristic X-rays produced by ion microbeam bombardment of a metal target. PIXEμCT was used to observe the body organs and internal structure of a living Drosophila melanogaster. Although the organs of the thorax were clearly imaged, the digestive organs in the abdominal cavity could not be clearly discerned initially, with the exception of the rectum and the Malpighian tubule. To enhance the abdominal images, a barium sulfate powder radiocontrast agent was added. For the first time, 3D images of the ventriculus of a living D. melanogaster were obtained. Our results showed that PIXEμCT can provide in vivo 3D-CT images that reflect correctly the structure of individual living organs, which is expected to be very useful in biological research.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume348
DOIs
Publication statusPublished - 2015

Keywords

  • 3D PIXE-micron-CT
  • Abdominal image
  • Contrast agent
  • Living Drosophila

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

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