In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold

Yingjun Wang; Xuetao Shi; Li Ren; Yongchang Yao; Dong An Wang

doi:10.1163/092050609X12481751806259

In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold

Yingjun Wang, Xuetao Shi, Li Ren, Yongchang Yao, Dong An Wang

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.

Original language	English
Pages (from-to)	1227-1238
Number of pages	12
Journal	Journal of Biomaterials Science, Polymer Edition
Volume	21
Issue number	8-9
DOIs	https://doi.org/10.1163/092050609X12481751806259
Publication status	Published - 2010 May 1
Externally published	Yes

Keywords

ALENDRONATE
DEXAMETHASONE
HYDROXYAPATITE
OSTEOGENESIS
POLY(LACTIC-CO-GLYCOLIC ACID)
SCAFFOLD

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Biomedical Engineering

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10.1163/092050609X12481751806259

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@article{85e5427c0e2146d6a66c260c9db19c3c,

title = "In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold",

abstract = "In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.",

keywords = "ALENDRONATE, DEXAMETHASONE, HYDROXYAPATITE, OSTEOGENESIS, POLY(LACTIC-CO-GLYCOLIC ACID), SCAFFOLD",

author = "Yingjun Wang and Xuetao Shi and Li Ren and Yongchang Yao and Wang, {Dong An}",

note = "Funding Information: Y. W. and X. S. contributed equally to this manuscript. X. S. is a China Scholarship Council (CSC) scholarship recipient (2007U33046). This research was supported by National Natural Science Foundation of China (Grants 50572029, 50732003, 50830101, U0834003 and 50803018), National Basic Research Program of China (2005CB623902), National Science and Technology Support Programme of China (2006BAI16B04), and Natural Science Foundation of Guangdong, China (4205786). The research was also supported by AcRF Tier 1 Grant RG64/08, Ministry of Education, Singapore.",

year = "2010",

month = may,

day = "1",

doi = "10.1163/092050609X12481751806259",

language = "English",

volume = "21",

pages = "1227--1238",

journal = "Journal of Biomaterials Science, Polymer Edition",

issn = "0920-5063",

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TY - JOUR

T1 - In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold

AU - Wang, Yingjun

AU - Shi, Xuetao

AU - Ren, Li

AU - Yao, Yongchang

AU - Wang, Dong An

N1 - Funding Information: Y. W. and X. S. contributed equally to this manuscript. X. S. is a China Scholarship Council (CSC) scholarship recipient (2007U33046). This research was supported by National Natural Science Foundation of China (Grants 50572029, 50732003, 50830101, U0834003 and 50803018), National Basic Research Program of China (2005CB623902), National Science and Technology Support Programme of China (2006BAI16B04), and Natural Science Foundation of Guangdong, China (4205786). The research was also supported by AcRF Tier 1 Grant RG64/08, Ministry of Education, Singapore.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.

AB - In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.

KW - ALENDRONATE

KW - DEXAMETHASONE

KW - HYDROXYAPATITE

KW - OSTEOGENESIS

KW - POLY(LACTIC-CO-GLYCOLIC ACID)

KW - SCAFFOLD

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VL - 21

SP - 1227

EP - 1238

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 8-9

ER -

In vitro osteogenesis of synovium mesenchymal cells induced by controlled release of alendronate and dexamethasone from a sintered microspherical scaffold

Abstract

Keywords

ASJC Scopus subject areas

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