Abstract
In vitro osteogenesis was successfully achieved with synovium-derived mesenchymal stem cells (SMSCs), which intrinsically have a strong chondrogenic tendency, by in situ release of alendronate (AL) and dexamethasone (Dex) from poly(lactic-co-glycolic acid) (PLGA)/hydroxyapatite (HA) sintered microspherical scaffold (PLGA/HA-SMS). Cumulative release profiles of AL and Dex from PLGA/HA-SMS and the influence on SMSCs osteogenic commitment were investigated. SMSCs seeded in Al-/Dex-loaded PLGA/HA-SMS (PLGA/HA-Com-SMS) exhibited significant osteogenic differentiation, as indicated by high yields of alkaline phosphatase (ALP) and bone calcification. In addition, mechanical properties (compressional) of PLGA/HA-Com-SMSs were also evaluated and approved. In conclusion, by promoting osteogenic commitment of SMSCs in vitro, this newly designed controlled-release system opens a new door to bone reparation and regeneration.
Original language | English |
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Pages (from-to) | 1227-1238 |
Number of pages | 12 |
Journal | Journal of Biomaterials Science, Polymer Edition |
Volume | 21 |
Issue number | 8-9 |
DOIs | |
Publication status | Published - 2010 May 1 |
Externally published | Yes |
Keywords
- ALENDRONATE
- DEXAMETHASONE
- HYDROXYAPATITE
- OSTEOGENESIS
- POLY(LACTIC-CO-GLYCOLIC ACID)
- SCAFFOLD
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Biomedical Engineering