In vitro molecular weight increase in xyloglucans by an apoplastic enzyme preparation from epicotyls of Vigna angularis

Kazuhiko Nishitani, Rumi Tominaga

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62 Citations (Scopus)

Abstract

In order to gain insight into the mechanism of cell extension growth, enzymic processes involved in structural modification of cell wall xyloglucans were investigated, using an apoplastic enzyme preparation from epicotyls of dark grown Vigna angularis Ohwi et Ohashi cv. Takara and purified xyloglucans derived from cell walls of Vigna. The reaction of Vigna xyloglucan (mass average molecular weight=420 kDa) with the apoplastic enzyme preparation gave three fractions: (1) a waterinsoluble high molecular weight (820 kDa) xyloglucan fraction (WI), (2) a watersoluble low molecular weight (149 kDa) xyloglucan fraction (WS), and (3) an 80% ethanol‐soluble monosaccharide fraction (ES). WI and WS were chiefly composed of t‐galactosyl‐, t‐xylosyl‐, 2‐xylosyl‐, 4‐glucosyl‐ and 4,6‐glucosyl residues, whereas ES was composed of fucose, galactose, glucose and xylose monomers. The data indicate that WI is generated by the linking of xyloglucan molecules by some alkali stable linkages, probably of glycosidic nature. The optimal pH for the WI‐producing activity of the apoplastic enzyme preparation was 5.4. Higher WI‐producing activity was detected in the upper juvenile than in the lower non‐elongating regions of the epicotyl. Our data suggest the possible involvement of a transglycosylation reaction in the structural changes of the xyloglucans that are responsible for cell extension growth of the Vigna angularis epicotyl. The data are also consistent with the idea that the enzymic processes are regulated by hydrogen ions in the apoplastic space.

Original languageEnglish
Pages (from-to)490-497
Number of pages8
JournalPhysiologia Plantarum
Volume82
Issue number4
DOIs
Publication statusPublished - 1991 Aug
Externally publishedYes

Keywords

  • Acid growth theory
  • Vigna angularis
  • apoplast
  • cell wall
  • transglycosylation
  • xyloglucan

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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