In vitro growth and differentiated activities of human periodontal ligament fibroblasts cultured on salmon collagen gel

Nobuhiro Nagai, Kazuo Mori, Yasuharu Satoh, Noritaka Takahashi, Shunji Yunoki, Kenji Tajima, Masanobu Munekata

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Marine-derived collagen is expected to be a much safer alternative to calf collagen, which in medical applications carries the risk of bovine spongiform encephalopathy. In this study, acid-soluble collagen was extracted from salmon skin and crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide during fibril formation to produce a crosslinked salmon collagen (SC) gel. The growth rates and the differentiated functions of human periodontal ligament fibroblasts (HPdLFs) cultured on the SC gel were investigated. Growth was faster on the SC gel than on porcine collagen (PC) gel. In addition, the HPdLFs cultured on the SC gel exhibited higher alkaline phosphatase (ALP) activity than those cultured on the PC gel. Quantitative RT-PCR revealed higher mRNA expression of type I collagen, ALP, and osteocalcin in the HPdLFs cultured on the SC gel. HPdLFs had a flat shape on the SC gel and a spindle shape on the PC gel, as revealed by observation with scanning electron microscopy and immunostaining with cytoskeletal protein and vinculin. The results showed that HPdLFs could grow and show highly differentiated activity on the SC gel as well as on the PC gel.

Original languageEnglish
Pages (from-to)395-402
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume82
Issue number2
DOIs
Publication statusPublished - 2007 Aug 1
Externally publishedYes

Keywords

  • Alkaline phophatase activity
  • Growth rate
  • Periodontal ligament fibroblasts
  • Quantitative RT-PCR
  • Salmon collagen

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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