In vitro detection of residual undifferentiated cells in retinal pigment epithelial cells derived from human induced pluripotent stem cells

Takuya Kuroda, Satoshi Yasuda, Yoji Sato

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are a leading candidate for regenerative medicine/cell therapies because of their capacity for pluripotency and unlimited self-renewal. However, there are significant obstacles preventing the clinical use of hPSCs. A significant safety issues is the presence of residual undifferentiated cells that have the potential to form tumors in vivo. Here, we describe the highly sensitive qRT-PCR methods for detection of residual undifferentiated cells in retinal pigment epithelial (RPE) cells derived from hiPSCs. qRT-PCR using probes and primers targeting LIN28A (LIN28) transcripts can detect residual undifferentiated cell levels as low as 0.002 % in hiPSC-derived RPE cells. We expect this method to contribute to process validation and quality control of hiPSC-derived cell therapy product.

Original languageEnglish
Pages (from-to)183-192
Number of pages10
JournalMethods in Molecular Biology
Volume1210
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • Human induced pluripotent stem cells
  • LIN28
  • QRT-PCR
  • Retinal pigment epithelial cells
  • Tumorigenicity

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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