In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30

Masaaki Izuma; Koju Kobayashi; Masaaki Shiina; Yoshiyuki Ueno; Motoyasu Ishii; Tooru Shimosegawa; Takayoshi Toyota; Kazuhiro Kakimi; Masaaki Miyazawa

doi:10.1016/S1386-6346(00)00071-1

In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30

Masaaki Izuma, Koju Kobayashi, Masaaki Shiina, Yoshiyuki Ueno, Motoyasu Ishii, Tooru Shimosegawa, Takayoshi Toyota, Kazuhiro Kakimi, Masaaki Miyazawa

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We investigated the responses of peripheral blood mononuclear cells (PBMCs) to hepatitis C virus core protein in ten patients with chronic hepatitis C during interferon (IFN)-β treatment to determine if the modulation of the immune reaction to hepatitis C virus by IFN treatment is associated with the viral clearance. Interleukin-2, interleukin-4, interleukin-10, and interferon-γ in the supernatant of the patients' PBMC co-cultured with the HCV core antigen-presenting autologous PBMCs were measured by ELISA. Serum levels of soluble CD (sCD) 8 and sCD30 in these patients were also measured by ELISA. The production of interleukin-2 and interferon-γ by PBMCs of sustained responders (SRs) increased after IFN-treatment, although it did not reach a significant level. Interleukin-10 was detected only in non-responders (NRs) at 0 and 4 weeks after the start of IFN treatment. Serum sCD8 level increased significantly in SR with IFN treatment. A close correlation between the serum sCD8 levels and interferon-γ levels in the supernatant at week 8 was observed in SR. These results suggest that IFN treatment potentiates the cellular immune reaction against HCV core protein more efficiently in SR than in NR. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

Original language	English
Pages (from-to)	218-229
Number of pages	12
Journal	Hepatology Research
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/S1386-6346(00)00071-1
Publication status	Published - 2000

Keywords

Hepatitis C
Interferon-γ
Interleukin-4
Sustained response

ASJC Scopus subject areas

Hepatology
Infectious Diseases

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Izuma, M., Kobayashi, K., Shiina, M., Ueno, Y., Ishii, M., Shimosegawa, T., Toyota, T., Kakimi, K., & Miyazawa, M. (2000). In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30. Hepatology Research, 18(3), 218-229. https://doi.org/10.1016/S1386-6346(00)00071-1

In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30. / Izuma, Masaaki; Kobayashi, Koju; Shiina, Masaaki; Ueno, Yoshiyuki; Ishii, Motoyasu; Shimosegawa, Tooru; Toyota, Takayoshi; Kakimi, Kazuhiro; Miyazawa, Masaaki.

In: Hepatology Research, Vol. 18, No. 3, 2000, p. 218-229.

Research output: Contribution to journal › Article › peer-review

Izuma, M, Kobayashi, K, Shiina, M, Ueno, Y, Ishii, M, Shimosegawa, T, Toyota, T, Kakimi, K & Miyazawa, M 2000, 'In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30', Hepatology Research, vol. 18, no. 3, pp. 218-229. https://doi.org/10.1016/S1386-6346(00)00071-1

Izuma, Masaaki ; Kobayashi, Koju ; Shiina, Masaaki ; Ueno, Yoshiyuki ; Ishii, Motoyasu ; Shimosegawa, Tooru ; Toyota, Takayoshi ; Kakimi, Kazuhiro ; Miyazawa, Masaaki. / In vitro cytokine production of peripheral blood mononuclear cells in response to HCV core antigen stimulation during interferon-β treatment and its relevance to sCD8 and sCD30. In: Hepatology Research. 2000 ; Vol. 18, No. 3. pp. 218-229.

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abstract = "We investigated the responses of peripheral blood mononuclear cells (PBMCs) to hepatitis C virus core protein in ten patients with chronic hepatitis C during interferon (IFN)-β treatment to determine if the modulation of the immune reaction to hepatitis C virus by IFN treatment is associated with the viral clearance. Interleukin-2, interleukin-4, interleukin-10, and interferon-γ in the supernatant of the patients' PBMC co-cultured with the HCV core antigen-presenting autologous PBMCs were measured by ELISA. Serum levels of soluble CD (sCD) 8 and sCD30 in these patients were also measured by ELISA. The production of interleukin-2 and interferon-γ by PBMCs of sustained responders (SRs) increased after IFN-treatment, although it did not reach a significant level. Interleukin-10 was detected only in non-responders (NRs) at 0 and 4 weeks after the start of IFN treatment. Serum sCD8 level increased significantly in SR with IFN treatment. A close correlation between the serum sCD8 levels and interferon-γ levels in the supernatant at week 8 was observed in SR. These results suggest that IFN treatment potentiates the cellular immune reaction against HCV core protein more efficiently in SR than in NR. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.",

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AU - Ueno, Yoshiyuki

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AU - Shimosegawa, Tooru

AU - Toyota, Takayoshi

AU - Kakimi, Kazuhiro

AU - Miyazawa, Masaaki

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AB - We investigated the responses of peripheral blood mononuclear cells (PBMCs) to hepatitis C virus core protein in ten patients with chronic hepatitis C during interferon (IFN)-β treatment to determine if the modulation of the immune reaction to hepatitis C virus by IFN treatment is associated with the viral clearance. Interleukin-2, interleukin-4, interleukin-10, and interferon-γ in the supernatant of the patients' PBMC co-cultured with the HCV core antigen-presenting autologous PBMCs were measured by ELISA. Serum levels of soluble CD (sCD) 8 and sCD30 in these patients were also measured by ELISA. The production of interleukin-2 and interferon-γ by PBMCs of sustained responders (SRs) increased after IFN-treatment, although it did not reach a significant level. Interleukin-10 was detected only in non-responders (NRs) at 0 and 4 weeks after the start of IFN treatment. Serum sCD8 level increased significantly in SR with IFN treatment. A close correlation between the serum sCD8 levels and interferon-γ levels in the supernatant at week 8 was observed in SR. These results suggest that IFN treatment potentiates the cellular immune reaction against HCV core protein more efficiently in SR than in NR. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

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