Cross-reactivities of synthetic lipid A analogues with monoclonal and conventional antibodies against Salmonella lipid A were studied. It was shown that the invitro antigenicity of a synthetic compound 506, β-(1→6) D-glucosamine disaccharide 1,4′-bisphosphate, which is acylated at 2′-amino and 3′-hydroxyl groups with (R)-3-dodecanoyloxytetradecanoyl and (R)-3-tetradecanoyloxytetradecanoyl groups, respectively, and has (R)-3-hydroxytetradecanoyl groups at 2-amino and 3-hydroxyl groups, was practically indistinguishable from that of the natural E. coli lipid A preparation, and that both phosphates in positions 1 and 4′ as well as ester- and amide-linked fatty acyl residues, particularly 3-acyloxyacyl group, of the glucosamine disaccharide are involved in the cross-reactivity of lipid A as important antigenic determinants.
|Number of pages||6|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1985 Apr 30|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology