In vitro antigenic reactivity of synthetic lipid a analogues as determined by monoclonal and conventional antibodies

Nobuhiko Kasai, Satoru Arata, Jun ichi Mashimo, Kenji Okuda, Yukoh Aihara, Shozo Kotani, Haruhiko Takada, Tetsuo Shiba, Shoichi Kusumoto

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Cross-reactivities of synthetic lipid A analogues with monoclonal and conventional antibodies against Salmonella lipid A were studied. It was shown that the invitro antigenicity of a synthetic compound 506, β-(1→6) D-glucosamine disaccharide 1,4′-bisphosphate, which is acylated at 2′-amino and 3′-hydroxyl groups with (R)-3-dodecanoyloxytetradecanoyl and (R)-3-tetradecanoyloxytetradecanoyl groups, respectively, and has (R)-3-hydroxytetradecanoyl groups at 2-amino and 3-hydroxyl groups, was practically indistinguishable from that of the natural E. coli lipid A preparation, and that both phosphates in positions 1 and 4′ as well as ester- and amide-linked fatty acyl residues, particularly 3-acyloxyacyl group, of the glucosamine disaccharide are involved in the cross-reactivity of lipid A as important antigenic determinants.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalBiochemical and biophysical research communications
Volume128
Issue number2
DOIs
Publication statusPublished - 1985 Apr 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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