In vitro activity of fluoroquinolone and the gyrA gene mutation in Helicobacter pylori strains isolated from children

Shigeru Fujimura, Seiichi Kato, Kazuie Iinuma, Akira Watanabe

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    47 Citations (Scopus)

    Abstract

    Resistance to antibiotics, especially clarithromycin, is the major cause of the failure to eradicate Helicobacter pylori. There are few studies in children concerning fluoroquinolone activity against H. pylori. Primary resistance to antibiotics including fluoroquinolones was studied in 55 H. pylori strains isolated from Japanese children. DNA sequences of the gyrA gene in fluoroquinolone-resistant strains were determined. Twelve strains (21.8%) were resistant to clarithromycin and three (5.5%) were resistant to both levofloxacin and ciprofloxacin. Out of 12 clarithromycin-resistant strains, 11 (91.7%) were susceptible to levofloxacin and ciprofloxacin. Sequence analysis in three fluoroquinolone-resistant strains showed point mutations of the gyrA gene at G271A, G271T and A272G, indicating mutations of the codon Asp91 in the fluoroquinolone-resistance-determining region of the DNA gyrase. The results suggest that fluoroquinolones should be considered as an option for second- or third-line H. pylori eradication therapy in children.

    Original languageEnglish
    Pages (from-to)1019-1022
    Number of pages4
    JournalJournal of Medical Microbiology
    Volume53
    Issue number10
    DOIs
    Publication statusPublished - 2004 Oct

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)

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