In situ real-time monitoring of biomolecular interactions by using surface infrared spectroscopy

Ayumi Hirano-Iwata, Ryo Taro Yamaguchi, Ko Ichiro Miyamoto, Yasuo Kimura, Michio Niwano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This paper reviews our recent approaches for in situ label-free detection of biomolecules and their interactions by using infrared absorption spectroscopy (IRAS) in the multiple internal reflection (MIR) geometry. Biomolecular interactions, such as DNA hybridization, DNA hydration, protein-protein interaction, cell growth and cell death, were characterized and monitored in situ both in D2O and H2O media. Combination of MIR-IRAS and various sensing platforms, such as Si, GaAs, porous Si, and porous amunina, was also discussed in terms of sensitivity and applicability to chip analysis. It is demonstrated that MIR-IRAS is a promising tool not only for the label-free detection of biomolecules but also for the accurate discrimination between specific and nonspecific interactions, which is critically important when we are monitoring complex and dynamic biological samples.

Original languageEnglish
Article number102039
JournalJournal of Applied Physics
Volume105
Issue number10
DOIs
Publication statusPublished - 2009 Jun 11

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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