In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy

Ko Ichiro Miyamoto, Parida Yamada, Ryo Taro Yamaguchi, Takami Muto, Ayumi Hirano, Yasuo Kimura, Michio Niwano, Hiroko Isoda

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37°C and a humidified gas mixture containing 5% CO2 was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm-1) and also by the absorption intensities of CH x bands (2,700-3,100 cm-1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.

Original languageEnglish
Pages (from-to)143-149
Number of pages7
JournalCytotechnology
Volume55
Issue number2-3
DOIs
Publication statusPublished - 2007 Dec

Keywords

  • Cell metabolism
  • Human breast cell line MCF-7
  • In situ observation
  • Infrared absorption spectroscopy
  • Si prisms

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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