Abstract
An in situ method for modifying a receptor site on mesoporous silica MCM-41 channels in planar lipid bilayers is described, in which bovine serum albumin (BSA) is covalently linked to the MCM-41 channels via head groups of lipids loaded in the nanopores. Prior to receptor modification, lipid-loaded MCM-41 channels were incorporated with lipid bilayers formed at an aperture of a Teflon film. The in situ coupling of BSA to lipid-loaded MCM-41 channels at the lipid bilayer interface was achieved by the sulfhydryl coupling method. The lipid bilayers containing BSA-modified MCM-41 exhibited channel-like currents, which were augmented in a concentration-dependent manner by the addition of anti-BSA at fM level. The in situ modification of lipid-loaded MCM-41 channels with BSA by the amine coupling technique was also investigated. The potential of the present approach for the development of channel-type biosensors is discussed in terms of modifying bilayer interfaces with bioreceptors.
Original language | English |
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Pages (from-to) | 139-144 |
Number of pages | 6 |
Journal | Sensors and Actuators, B: Chemical |
Volume | 160 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2011 Dec 15 |
Keywords
- Anti-BSA antibody
- Bovine serum albumin
- In situ modification of bioreceptor
- Lipid bilayer
- MCM-41 channel
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
- Electrical and Electronic Engineering