In situ modification of lipid-loaded MCM-41 channels with bovine serum albumin at a planar lipid bilayer for biosensing

Keiichiro Nozawa, Azusa Oshima, Tomohiro Nasu, Atsushi Shoji, Ayumi Hirano, Michio Niwano, Masao Sugawara

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

An in situ method for modifying a receptor site on mesoporous silica MCM-41 channels in planar lipid bilayers is described, in which bovine serum albumin (BSA) is covalently linked to the MCM-41 channels via head groups of lipids loaded in the nanopores. Prior to receptor modification, lipid-loaded MCM-41 channels were incorporated with lipid bilayers formed at an aperture of a Teflon film. The in situ coupling of BSA to lipid-loaded MCM-41 channels at the lipid bilayer interface was achieved by the sulfhydryl coupling method. The lipid bilayers containing BSA-modified MCM-41 exhibited channel-like currents, which were augmented in a concentration-dependent manner by the addition of anti-BSA at fM level. The in situ modification of lipid-loaded MCM-41 channels with BSA by the amine coupling technique was also investigated. The potential of the present approach for the development of channel-type biosensors is discussed in terms of modifying bilayer interfaces with bioreceptors.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume160
Issue number1
DOIs
Publication statusPublished - 2011 Dec 15

Keywords

  • Anti-BSA antibody
  • Bovine serum albumin
  • In situ modification of bioreceptor
  • Lipid bilayer
  • MCM-41 channel

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry
  • Electrical and Electronic Engineering

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