TY - JOUR
T1 - In situ expression of the cell adhesion molecules in inflammatory bowel disease
T2 - Evidence of immunologic activation of vascular endothelial cells
AU - Nakamura, S.
AU - Ohtani, H.
AU - Watanabe, Y.
AU - Fukushima, K.
AU - Matsumoto, T.
AU - Kitano, A.
AU - Kobayashi, K.
AU - Nagura, H.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - BACKGROUND: The cell adhesion between vascular endothelial cells and leukocytes is an important process for the immuno-inflammatory changes. To clarify the basic features of inflammatory bowel disease (IBD), studies of in situ localization of the cell adhesion molecules are required. EXPERIMENTAL DESIGN: We analyzed the immunohistochemical localization of the adhesion molecules (ICAM-1, LFA-1, Mac-1, VCAM-1, VLA-4, P- and E-selectins) in IBD, stressing phenotypical changes of endothelial cells. RESULTS: In the normal mucosa, ICAM-1 was expressed in capillaries and venules, LFA-1 in some lymphocytes and VLA-4 in most lymphocytes. VCAM-1 was expressed sporadically in venules and constantly in follicular dendritic cells (FDC) in lymphoid follicles. Both E- and P-selectins were sporadically expressed in venules. In actively inflamed mucosa in IBD, a marked increase of all these antigens was observed; ICAM-1+ inflammatory infiltrates (lymphocytes, plasma cells, and macrophages) and ICAM-1+ venules increased paralleled to the degree of inflammation. LFA-1+ and VLA-4+ mononuclear cells and Mac-1+ granulocytes increased in number. However, expression of VCAM-1 in venules or capillaries was not increased. FDC constantly expressed VCAM-1. E- and P-selectins+ venules increased in actively inflamed tissue particularly at the base of ulcers. Immunoelectron microscopy confirmed expression of these antigens along the plasma membranes (functional localization) and in rough endoplasmic reticulum or in perinuclear spaces (localization during the intracellular synthesis). CONCLUSIONS: Our study suggested that increased expression of the adhesion molecules in IBD promotes the recruitment of granulocytes and lymphocytes through blood vessels and the cell interaction between lymphocytes-antigen presenting cells or among lymphocytes, thereby sustaining the immuno-inflammatory process in IBD. The present study emphasizes the importance of microenvironmental changes including the endothelial activation in the immuno-inflammatory changes.
AB - BACKGROUND: The cell adhesion between vascular endothelial cells and leukocytes is an important process for the immuno-inflammatory changes. To clarify the basic features of inflammatory bowel disease (IBD), studies of in situ localization of the cell adhesion molecules are required. EXPERIMENTAL DESIGN: We analyzed the immunohistochemical localization of the adhesion molecules (ICAM-1, LFA-1, Mac-1, VCAM-1, VLA-4, P- and E-selectins) in IBD, stressing phenotypical changes of endothelial cells. RESULTS: In the normal mucosa, ICAM-1 was expressed in capillaries and venules, LFA-1 in some lymphocytes and VLA-4 in most lymphocytes. VCAM-1 was expressed sporadically in venules and constantly in follicular dendritic cells (FDC) in lymphoid follicles. Both E- and P-selectins were sporadically expressed in venules. In actively inflamed mucosa in IBD, a marked increase of all these antigens was observed; ICAM-1+ inflammatory infiltrates (lymphocytes, plasma cells, and macrophages) and ICAM-1+ venules increased paralleled to the degree of inflammation. LFA-1+ and VLA-4+ mononuclear cells and Mac-1+ granulocytes increased in number. However, expression of VCAM-1 in venules or capillaries was not increased. FDC constantly expressed VCAM-1. E- and P-selectins+ venules increased in actively inflamed tissue particularly at the base of ulcers. Immunoelectron microscopy confirmed expression of these antigens along the plasma membranes (functional localization) and in rough endoplasmic reticulum or in perinuclear spaces (localization during the intracellular synthesis). CONCLUSIONS: Our study suggested that increased expression of the adhesion molecules in IBD promotes the recruitment of granulocytes and lymphocytes through blood vessels and the cell interaction between lymphocytes-antigen presenting cells or among lymphocytes, thereby sustaining the immuno-inflammatory process in IBD. The present study emphasizes the importance of microenvironmental changes including the endothelial activation in the immuno-inflammatory changes.
KW - Cell adhesion molecule
KW - Endothelial cell
KW - Immunohistochemistry
KW - Inflammatory bowel disease
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M3 - Article
C2 - 7687311
AN - SCOPUS:0027179524
VL - 69
SP - 77
EP - 85
JO - Laboratory Investigation
JF - Laboratory Investigation
SN - 0023-6837
IS - 1
ER -