In situ expression of fibrogenic growth factors and their receptors in biliary atresia: Comparison between early and late stages

Abul Faiz Kabir Uddin Ahmed, Haruo Ohtani, Masaki Nio, Nobuo Funaki, Daiji Iwami, Shinji Kumagai, Eiichi Sato, Hiroshi Nagura, Ryoji Ohi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Progressive fibrosis, despite successful surgical treatment, is one of the serious complications of biliary atresia. To understand the mechanism of this fibrosis, the in situ expression of fibrogenic growth factors (TGF-β and PDGF) and their corresponding receptors was studied by immunohistochemistry using frozen sections. The results were compared between the early (n = 12) and late (n = 6) stages. The early stage was characterized by abundant expression of all ligands and receptors, together with type I procollagen (PC-I). The major cellular sources were activated fibroblasts/myofibroblasts distributed mostly in the portal tracts. Macrophages also expressed all the ligands and the receptors, but to a lesser degree. Bile duct cells strongly expressed TGF-β RI and RII and PDGF AA and BB, but focally expressed TGF-β. All of these decreased in the late stage of biliary atresia. These results suggest that TGF-β and PDGF play important roles in the fibrogenesis of biliary atresia, especially in its early stage, acting either by autocrine or paracrine mechanisms involving activated fibroblasts/myofibroblasts, bile duct cells, and macrophages. (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalJournal of Pathology
Volume192
Issue number1
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Biliary atresia
  • Fibrogenesis
  • Growth factor
  • PDGF
  • Procollagen
  • TGF-β

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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