In silico predicted structural and functional insights of all missense mutations on 2B domain of K1/K10 causing genodermatoses

Santasree Banerjee, Qian Wu, Yuyi Ying, Yanni Li, Matsuyuki Shirota, Dante Neculai, Chen Li

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The K1 and K10 associated genodermatoses are characterized by clinical symptoms of mild to severe redness, blistering and hypertrophy of the skin. In this paper, we set out to computationally investigate the structural and functional effects of missense mutations on the 2B domain of K1/K10 heterodimer and its consequences in disease phenotype. We modeled the structure of the K1/K10 heterodimer based on crystal structures for the human homolog K5/K14 heterodimer, and identified that the missense mutations exert their effects on stability and assembly competence of the heterodimer by altering physico-chemical properties, interatomic interactions, and inter-residue atomic contacts. Comparative structural analysis between all the missense mutations and SNPs showed that the location and physico-chemical properties of the substituted amino acid are significantly correlated with phenotypic variations. In particular, we find evidence that a particular SNP (K10, p.E443K) is a pathogenic nsSNP which disrupts formation of the hydrophobic core and destabilizes the heterodimer through the loss of interatomic interactions. Our study is the first comprehensive report analyzing the mutations located on 2B domain of K1/K10 heterodimeric coiled-coil complex.

Original languageEnglish
Pages (from-to)52766-52780
Number of pages15
JournalOncotarget
Volume7
Issue number33
DOIs
Publication statusPublished - 2016 Aug 1

Keywords

  • Coiled-coil heterodimer
  • Genodermatoses
  • In silico analysis
  • Missense mutations

ASJC Scopus subject areas

  • Oncology

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