In-one-pot-at-a-time ligation for high-throughput construction of a protein expression vector library

Hikaru Nakazawa, Rui Todokoro, Yuri Ishigaki, Izumi Kumagai, Mitsuo Umetsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A mixture of protein-encoding gene fragments was ligated into linearized expression vectors in one pot at a time (iPaT) to simultaneously generate each protein expression vector. This high-throughput iPaT ligation method was stable and highly efficient for the simultaneous generation of more than 10 different expression vectors.

Original languageEnglish
Pages (from-to)424-426
Number of pages3
JournalChemistry Letters
Volume42
Issue number4
DOIs
Publication statusPublished - 2013 Apr 15

ASJC Scopus subject areas

  • Chemistry(all)

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