TY - JOUR
T1 - Improving the viability of tissue-resident stem cells using an organ-preservation solution
AU - Suzuki, Takaya
AU - Ota, Chiharu
AU - Fujino, Naoya
AU - Tando, Yukiko
AU - Suzuki, Satoshi
AU - Yamada, Mitsuhiro
AU - Kondo, Takashi
AU - Okada, Yoshinori
AU - Kubo, Hiroshi
N1 - Funding Information:
We greatly acknowledge the staff of the Biomedical Research Core of Tohoku University Graduate School of Medicine. This study was supported by Japan Society for the Promotion of Science (Grants 19390222, 22390163 and 25293190 to HK).
Publisher Copyright:
© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Human clinical specimens are a valuable source of tissue-resident stem cells, but such cells need to be collected immediately after tissue collection. To extend the timescale for collection from fresh human samples, we developed a new extracellular fluid (ECF)-type preservation solution based on a high-sodium and low-potassium solution containing low-molecular-weight dextran and glucose, which is used for preservation of organs for transplantation. In this study, we compared the preservation of tissue-resident stem cells using our ECF solution with that using three other solutions: PBS, Dulbecco’s modified Eagle’s medium and Euro-Collins solution. These solutions represent a common buffer, a common culture medium and a benchmark organ-preservation solution, respectively. Lung tissues were removed from mice and preserved for 72 h under low-temperature conditions. Of the solutions tested, only preservation in the ECF-type solution could maintain the proliferation and differentiation capacity of mouse lung tissue-resident stem cells. In addition, the ECF solution could preserve the viability and proliferation of human alveolar epithelial progenitor cells when stored for more than 7 days at 4 °C. The mean viability of human alveolar type II cells at 2, 5, 8 and 14 days of low-temperature preservation was 90.9%, 84.8%, 85.7% and 66.3%, respectively, with no significant differences up to 8 days. Overall, our findings show that use of our ECF-type preservation solution may maintain the viability and function of tissue-resident stem cells. Use of this preservation solution may facilitate the investigation of currently unobtainable human tissue specimens for human stem cell biology.
AB - Human clinical specimens are a valuable source of tissue-resident stem cells, but such cells need to be collected immediately after tissue collection. To extend the timescale for collection from fresh human samples, we developed a new extracellular fluid (ECF)-type preservation solution based on a high-sodium and low-potassium solution containing low-molecular-weight dextran and glucose, which is used for preservation of organs for transplantation. In this study, we compared the preservation of tissue-resident stem cells using our ECF solution with that using three other solutions: PBS, Dulbecco’s modified Eagle’s medium and Euro-Collins solution. These solutions represent a common buffer, a common culture medium and a benchmark organ-preservation solution, respectively. Lung tissues were removed from mice and preserved for 72 h under low-temperature conditions. Of the solutions tested, only preservation in the ECF-type solution could maintain the proliferation and differentiation capacity of mouse lung tissue-resident stem cells. In addition, the ECF solution could preserve the viability and proliferation of human alveolar epithelial progenitor cells when stored for more than 7 days at 4 °C. The mean viability of human alveolar type II cells at 2, 5, 8 and 14 days of low-temperature preservation was 90.9%, 84.8%, 85.7% and 66.3%, respectively, with no significant differences up to 8 days. Overall, our findings show that use of our ECF-type preservation solution may maintain the viability and function of tissue-resident stem cells. Use of this preservation solution may facilitate the investigation of currently unobtainable human tissue specimens for human stem cell biology.
KW - mesenchymal stem cell
KW - organ preservation
KW - preservation solution
KW - progenitor cell
KW - stem cell
KW - tissue transport
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U2 - 10.1002/2211-5463.12748
DO - 10.1002/2211-5463.12748
M3 - Article
C2 - 31642604
AN - SCOPUS:85075418933
VL - 9
SP - 2093
EP - 2104
JO - FEBS Open Bio
JF - FEBS Open Bio
SN - 2211-5463
IS - 12
ER -