TY - JOUR
T1 - Improving biohydrogen production through dark fermentation of steam-heated acid pretreated Alternanthera philoxeroides by mutant Enterobacter aerogenes ZJU1
AU - Song, Wenlu
AU - Ding, Lingkan
AU - Liu, Min
AU - Cheng, Jun
AU - Zhou, Junhu
AU - Li, Yu You
N1 - Funding Information:
This study was supported by the National Key Research and Development Program-China (2016YFE0117900), Shandong Provincial Natural Science Foundation-China (ZR2019MC060), Zhejiang Provincial Key Research and Development Program-China (2017C04001), and Key Research and Development Program of Jining City-China (2018ZDGH024).
Funding Information:
This study was supported by the National Key Research and Development Program -China ( 2016YFE0117900 ), Shandong Provincial Natural Science Foundation -China ( ZR2019MC060 ), Zhejiang Provincial Key Research and Development Program-China (2017C04001), and Key Research and Development Program of Jining City-China (2018ZDGH024).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/5/10
Y1 - 2020/5/10
N2 - Alternanthera philoxeroides, a notorious invasive aquatic weed, is a typical lignocellulosic feedstock for fermentative biohydrogen production. To improve the dark fermentation performance, steam-heated acid pretreatment and enzymolysis were employed to release reducing sugars from A. philoxeroides, and Enterobacter aerogenes ZJU1 mutagenized by 60Co-γ irradiation was used as the inoculum. Dilute acid accompanied by steam heating significantly disrupted the fiber structures of A. philoxeroides. Scanning electron microscopic images revealed that many pores and fissures were generated in the surface of A. philoxeroides after pretreatment. X-ray diffraction and Fourier transform infrared spectroscopy analyses showed that the pretreatment facilitated the transformation of cellulose I to cellulose II in A. philoxeroides biomass, resulting in the increase of amorphous regions and the decrease of crystallinity. Under the optimum pretreatment condition (1.0 v/v% H2SO4, 135 °C for 15 min), the reducing sugar yield reached 0.354 g/g A. philoxeroides, which was further increased to 0.575 g/g A. philoxeroides after enzymolysis. The biohydrogen yield increased by 59.9% from 38.9 mL/g volatile solids (VS) of raw A. philoxeroides to 62.2 mL/gVS of the pretreated one. As compared to the wild strain, E. aerogenes ZJU1 contributed to an increase of 31.8% in the biohydrogen yield from pretreated A. philoxeroides. Further optimization of bacteria suspensions significantly increased the maximum biohydrogen production rate from 1.42 to 4.64 mL/gVS/h, advanced the biohydrogen production peak, and resulted in an increase of 42.8% in biohydrogen yield to 89.8 mL/gVS.
AB - Alternanthera philoxeroides, a notorious invasive aquatic weed, is a typical lignocellulosic feedstock for fermentative biohydrogen production. To improve the dark fermentation performance, steam-heated acid pretreatment and enzymolysis were employed to release reducing sugars from A. philoxeroides, and Enterobacter aerogenes ZJU1 mutagenized by 60Co-γ irradiation was used as the inoculum. Dilute acid accompanied by steam heating significantly disrupted the fiber structures of A. philoxeroides. Scanning electron microscopic images revealed that many pores and fissures were generated in the surface of A. philoxeroides after pretreatment. X-ray diffraction and Fourier transform infrared spectroscopy analyses showed that the pretreatment facilitated the transformation of cellulose I to cellulose II in A. philoxeroides biomass, resulting in the increase of amorphous regions and the decrease of crystallinity. Under the optimum pretreatment condition (1.0 v/v% H2SO4, 135 °C for 15 min), the reducing sugar yield reached 0.354 g/g A. philoxeroides, which was further increased to 0.575 g/g A. philoxeroides after enzymolysis. The biohydrogen yield increased by 59.9% from 38.9 mL/g volatile solids (VS) of raw A. philoxeroides to 62.2 mL/gVS of the pretreated one. As compared to the wild strain, E. aerogenes ZJU1 contributed to an increase of 31.8% in the biohydrogen yield from pretreated A. philoxeroides. Further optimization of bacteria suspensions significantly increased the maximum biohydrogen production rate from 1.42 to 4.64 mL/gVS/h, advanced the biohydrogen production peak, and resulted in an increase of 42.8% in biohydrogen yield to 89.8 mL/gVS.
KW - Alternanthera philoxeroides
KW - Biohydrogen
KW - Dark fermentation
KW - Mutant Enterobacter aerogenes ZJU1
KW - Steam-heated acid pretreatment
UR - http://www.scopus.com/inward/record.url?scp=85076547057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076547057&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2019.134695
DO - 10.1016/j.scitotenv.2019.134695
M3 - Article
C2 - 31837880
AN - SCOPUS:85076547057
VL - 716
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 134695
ER -