Improvement of the transformation efficiency of Sacchaaromyces cerevisiae by altering carbon sources in pre-culture

Tatsunori Konishi, Masahiko Harata

Research output: Contribution to journalArticle

Abstract

We show here that the transformation efficiency of Saccharomyces cerevisiae is improved by altering carbon sources in media for pre-culturing cells prior to the transformation reactions. The transformation efficiency was increased up to sixfold by combination with existing transformation protocols. This method is widely applicable for yeast research since efficient transformation can be performed easily without changing any of the other procedures in the transformation.

Original languageEnglish
Pages (from-to)1090-1093
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • High-efficient transformation
  • Low-glucose medium
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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