Improvement of the transformation efficiency of Sacchaaromyces cerevisiae by altering carbon sources in pre-culture

Tatsunori Konishi, Masahiko Harata

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    We show here that the transformation efficiency of Saccharomyces cerevisiae is improved by altering carbon sources in media for pre-culturing cells prior to the transformation reactions. The transformation efficiency was increased up to sixfold by combination with existing transformation protocols. This method is widely applicable for yeast research since efficient transformation can be performed easily without changing any of the other procedures in the transformation.

    Original languageEnglish
    Pages (from-to)1090-1093
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume78
    Issue number6
    DOIs
    Publication statusPublished - 2014

    Keywords

    • High-efficient transformation
    • Low-glucose medium
    • Saccharomyces cerevisiae

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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