Improvement of sensitivity of the light-addressable potentiometric sensors for the purpose of noninvasive measurement of electrical activity of biological cells

A. B.Md Ismail, H. Sugihara, T. Yukimasa, T. Yoshinobu, H. Iwasaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The possibility of measurement of the electrical activity of neurons by Light-Addressable Potentiometric Sensor (LAPS) is investigated in this article. For the measurement of the extracellular transient of the neural action potential, which is very low, at the surface of a LAPS heterostructure, a simple structure like SiO2/Si, instead of the conventional Si3N4/SiO2/Si heterostructure, is studied. The SiO2 surface was modified by Poly-L-Ornithine and Laminin (PLOL). Experimental results show that the PLOL coating on the SiO2 surface not only prevents the hydration of the SiO2 layer and thus enables us to use SiO2 only as the insulating layer, but also, provides an efficient culturing of neurons of Lymnaea stagnalis, a kind of sea snail, on the SiO2 surface. The PLOL modified SiO2 layer of the novel LAPS heterostructure, is kept in contact with the neuron-culture medium for ten days mimicking the maximum cell-culturing period. There is no appreciable change in the sensor response was observed during this period. A DC bias, superimposed by a 2 mVp-p and 800 Hz AC was applied to the sensor to simulate an extracellular transient of 2 mVp-p to the surface and the response of the sensor shows the possibility of the measurement of the neural action potential by the LAPS.

Original languageEnglish
Title of host publicationSIcon 2001 - Proceedings of the 1st ISA/IEEE Sensors for Industry Conference
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages215-218
Number of pages4
ISBN (Electronic)078036659X, 9780780366596
DOIs
Publication statusPublished - 2001
Externally publishedYes
Event1st ISA/IEEE Sensors for Industry Conference, SIcon 2001 - Rosemont, United States
Duration: 2001 Nov 52001 Nov 7

Publication series

NameSIcon 2001 - Proceedings of the 1st ISA/IEEE Sensors for Industry Conference

Other

Other1st ISA/IEEE Sensors for Industry Conference, SIcon 2001
CountryUnited States
CityRosemont
Period01/11/501/11/7

ASJC Scopus subject areas

  • Instrumentation
  • Signal Processing
  • Electrical and Electronic Engineering
  • Industrial and Manufacturing Engineering

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