TY - JOUR
T1 - Improvement of mitochondrial DNA haplotyping in Japanese ‰ounder populations using the sequences of control region and ND2 gene
AU - Ando, Daiki
AU - Ikeda, Minoru
AU - Sekino, Masashi
AU - Sugaya, Takuma
AU - Katamachi, Daisuke
AU - Yoseda, Kenzo
AU - Kijima, Akihiro
PY - 2016
Y1 - 2016
N2 - In mitochondrial (mt) DNA haplotyping for populations of Japanese ‰ounder, the nucleotide sequences of the rst-half control region (FH-CR) have been frequently used because of the high sequence variability.We studied the possibility of improving the accuracy of mtDNA haplotyping by adding sequence data of ND2 and ND5 genes. The nest resolution of haplotypes was obtained when concatenated sequences of FH-CR and ND2 were used. Thus, mtDNA haplotyping based on the simultaneous use of the two mtDNA regions will provide an effcient means to precisely evaluate the genetic diversity of Japanese ‰ounder in relation to the stock enhancement program.
AB - In mitochondrial (mt) DNA haplotyping for populations of Japanese ‰ounder, the nucleotide sequences of the rst-half control region (FH-CR) have been frequently used because of the high sequence variability.We studied the possibility of improving the accuracy of mtDNA haplotyping by adding sequence data of ND2 and ND5 genes. The nest resolution of haplotypes was obtained when concatenated sequences of FH-CR and ND2 were used. Thus, mtDNA haplotyping based on the simultaneous use of the two mtDNA regions will provide an effcient means to precisely evaluate the genetic diversity of Japanese ‰ounder in relation to the stock enhancement program.
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U2 - 10.2331/suisan.16-00025
DO - 10.2331/suisan.16-00025
M3 - Article
AN - SCOPUS:84991358589
VL - 82
SP - 712
EP - 719
JO - Nippon Suisan Gakkaishi
JF - Nippon Suisan Gakkaishi
SN - 0021-5392
IS - 5
ER -