Improvement of mitochondrial DNA haplotyping in Japanese ‰ounder populations using the sequences of control region and ND2 gene

In mitochondrial (mt) DNA haplotyping for populations of Japanese ‰ounder, the nucleotide sequences of the rst-half control region (FH-CR) have been frequently used because of the high sequence variability.We studied the possibility of improving the accuracy of mtDNA haplotyping by adding sequence data of ND2 and ND5 genes. The nest resolution of haplotypes was obtained when concatenated sequences of FH-CR and ND2 were used. Thus, mtDNA haplotyping based on the simultaneous use of the two mtDNA regions will provide an effcient means to precisely evaluate the genetic diversity of Japanese ‰ounder in relation to the stock enhancement program.