Improvement of Hen Egg White Lysozyme Crystal Quality by Control of Dehydration Process

Haruhiko Koizumi; Satoshi Uda; Katsuo Tsukamoto; Kenji Hanada; Ryo Suzuki; Masaru Tachibana; Kenichi Kojima

doi:10.1021/acs.cgd.9b01084

Improvement of Hen Egg White Lysozyme Crystal Quality by Control of Dehydration Process

Haruhiko Koizumi, Satoshi Uda, Katsuo Tsukamoto, Kenji Hanada, Ryo Suzuki, Masaru Tachibana, Kenichi Kojima

Materials Development Division

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Equal-thickness fringes that are attributed to the Pendellösung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10^-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10^-4°.

Original language	English
Pages (from-to)	5955-5960
Number of pages	6
Journal	Crystal Growth and Design
Volume	19
Issue number	10
DOIs	https://doi.org/10.1021/acs.cgd.9b01084
Publication status	Published - 2019 Oct 2

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Condensed Matter Physics

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title = "Improvement of Hen Egg White Lysozyme Crystal Quality by Control of Dehydration Process",

abstract = "Equal-thickness fringes that are attributed to the Pendell{\"o}sung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10-4°.",

author = "Haruhiko Koizumi and Satoshi Uda and Katsuo Tsukamoto and Kenji Hanada and Ryo Suzuki and Masaru Tachibana and Kenichi Kojima",

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T1 - Improvement of Hen Egg White Lysozyme Crystal Quality by Control of Dehydration Process

AU - Koizumi, Haruhiko

AU - Uda, Satoshi

AU - Tsukamoto, Katsuo

AU - Hanada, Kenji

AU - Suzuki, Ryo

AU - Tachibana, Masaru

AU - Kojima, Kenichi

PY - 2019/10/2

Y1 - 2019/10/2

N2 - Equal-thickness fringes that are attributed to the Pendellösung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10-4°.

AB - Equal-thickness fringes that are attributed to the Pendellösung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10-4°.

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