TY - JOUR
T1 - Improvement of Hen Egg White Lysozyme Crystal Quality by Control of Dehydration Process
AU - Koizumi, Haruhiko
AU - Uda, Satoshi
AU - Tsukamoto, Katsuo
AU - Hanada, Kenji
AU - Suzuki, Ryo
AU - Tachibana, Masaru
AU - Kojima, Kenichi
N1 - Funding Information:
The authors thank Dr. H. Sugiyama and Dr. K. Hirano of KEK for their help with XRD rocking-curve measurements. XRD rocking-curve measurements were performed at the Photon Factory under the auspices of the Photon Factory Program Advisory Committee of KEK (Proposal No. 2018G547). This work was carried out under the GIMRT Program of the Institute for Materials Research, Tohoku University (Proposal Nos. 18K0109 and 19K0029). This work was supported in part by a Kakenhi Grant-in-Aid for Scientific Research (No. 16K06708) from the Japan Society for the Promotion of Science (JSPS).
Funding Information:
The authors thank Dr. H. Sugiyama and Dr. K. Hirano of KEK for their help with XRD rocking-curve measurements. XRD rocking-curve measurements were performed at the Photon Factory under the auspices of the Photon Factory Program Advisory Committee of KEK (Proposal No. 2018G547). This work was carried out under the GIMRT Program of the Institute for Materials Research, Tohoku University (Proposal Nos. 18K0109 and 19K0029). This work was supported in part by a Kakenhi Grant-in-Aid for Scientific Research (No. 16K06708) from the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/10/2
Y1 - 2019/10/2
N2 - Equal-thickness fringes that are attributed to the Pendellösung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10-4°.
AB - Equal-thickness fringes that are attributed to the Pendellösung effect were clearly observed in the region of a tapered tetragonal hen egg white (HEW) lysozyme crystal with a wedgelike edge, which indicates that the misorientation between subgrains was on the order of 10-4°. This is attributed to control of the dehydration process for crystal growth from solution, which plays an important role. Both the local quality and whole homogeneity of the crystals were improved by regulating the dehydration process. Oscillatory contrast was observed in X-ray topographs from regions of the same thickness in the crystals. However, the area of such a contrast was quite small, which indicates the presence of defects, even in crystals for which equal-thickness fringes could be observed. We also discuss why the area of oscillatory contrast was small for tetragonal HEW lysozyme crystals with misorientations between subgrains on the order of 10-4°.
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U2 - 10.1021/acs.cgd.9b01084
DO - 10.1021/acs.cgd.9b01084
M3 - Article
AN - SCOPUS:85072953236
VL - 19
SP - 5955
EP - 5960
JO - Crystal Growth and Design
JF - Crystal Growth and Design
SN - 1528-7483
IS - 10
ER -