Improvement of enzymatic activity of β-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate

Motonori Kudou; Yuka Kubota; Nanami Nakashima; Fumiyoshi Okazaki; Kazunori Nakashima; Chiaki Ogino; Akihiko Kondo

doi:10.1016/j.molcatb.2014.02.013

Improvement of enzymatic activity of β-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate

Motonori Kudou, Yuka Kubota, Nanami Nakashima, Fumiyoshi Okazaki, Kazunori Nakashima, Chiaki Ogino, Akihiko Kondo

ENG - Chemical Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The uses of ionic liquids (ILs) to alter the performance of enzymes and to pretreat cellulosic biomass have been investigated. In the present study, the activity of β-glucosidase from Themotoga maritima (TmBgl1A) was studied in a phosphate buffer containing imidazolium-based ILs. Among the imidazolium-based ILs that were tested, 1-butyl-3-methylimidazolium acetate [Bmim][OAc] showed the most improvement for the hydrolysis activity of TmBgl1A at pH 7.0. From the steady-state emission spectra, [Bmim][OAc] quenched the intrinsic fluorescence of TmBgl1A. The improvement in the activity of TmBgl1A induced by [Bmim][OAc] could be linked to the flexibility of the conformation of TmBgl1A.

Original language	English
Pages (from-to)	17-22
Number of pages	6
Journal	Journal of Molecular Catalysis B: Enzymatic
Volume	104
DOIs	https://doi.org/10.1016/j.molcatb.2014.02.013
Publication status	Published - 2014 Jun

Keywords

Bioprocess
Cellulose
Enzymology
Ionic liquid
β-Glucosidase

ASJC Scopus subject areas

Catalysis
Bioengineering
Biochemistry
Process Chemistry and Technology

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Improvement of enzymatic activity of β-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate. / Kudou, Motonori; Kubota, Yuka; Nakashima, Nanami; Okazaki, Fumiyoshi; Nakashima, Kazunori; Ogino, Chiaki; Kondo, Akihiko.

In: Journal of Molecular Catalysis B: Enzymatic, Vol. 104, 06.2014, p. 17-22.

Research output: Contribution to journal › Article › peer-review

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title = "Improvement of enzymatic activity of β-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate",

abstract = "The uses of ionic liquids (ILs) to alter the performance of enzymes and to pretreat cellulosic biomass have been investigated. In the present study, the activity of β-glucosidase from Themotoga maritima (TmBgl1A) was studied in a phosphate buffer containing imidazolium-based ILs. Among the imidazolium-based ILs that were tested, 1-butyl-3-methylimidazolium acetate [Bmim][OAc] showed the most improvement for the hydrolysis activity of TmBgl1A at pH 7.0. From the steady-state emission spectra, [Bmim][OAc] quenched the intrinsic fluorescence of TmBgl1A. The improvement in the activity of TmBgl1A induced by [Bmim][OAc] could be linked to the flexibility of the conformation of TmBgl1A.",

keywords = "Bioprocess, Cellulose, Enzymology, Ionic liquid, β-Glucosidase",

author = "Motonori Kudou and Yuka Kubota and Nanami Nakashima and Fumiyoshi Okazaki and Kazunori Nakashima and Chiaki Ogino and Akihiko Kondo",

note = "Funding Information: This work was supported by the New Energy and Industrial Technology Development Organization (The Elemental Bioenergy Conversion Technologies and Related Peripheral Elemental Technologies Project) of Japan (no. 09005647 to C.O.), and by Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan. ",

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AU - Okazaki, Fumiyoshi

AU - Nakashima, Kazunori

AU - Ogino, Chiaki

AU - Kondo, Akihiko

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KW - Enzymology

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