Abstract
The uses of ionic liquids (ILs) to alter the performance of enzymes and to pretreat cellulosic biomass have been investigated. In the present study, the activity of β-glucosidase from Themotoga maritima (TmBgl1A) was studied in a phosphate buffer containing imidazolium-based ILs. Among the imidazolium-based ILs that were tested, 1-butyl-3-methylimidazolium acetate [Bmim][OAc] showed the most improvement for the hydrolysis activity of TmBgl1A at pH 7.0. From the steady-state emission spectra, [Bmim][OAc] quenched the intrinsic fluorescence of TmBgl1A. The improvement in the activity of TmBgl1A induced by [Bmim][OAc] could be linked to the flexibility of the conformation of TmBgl1A.
| Original language | English |
|---|---|
| Pages (from-to) | 17-22 |
| Number of pages | 6 |
| Journal | Journal of Molecular Catalysis B: Enzymatic |
| Volume | 104 |
| DOIs | |
| Publication status | Published - 2014 Jun |
Keywords
- Bioprocess
- Cellulose
- Enzymology
- Ionic liquid
- β-Glucosidase
ASJC Scopus subject areas
- Catalysis
- Bioengineering
- Biochemistry
- Process Chemistry and Technology
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Cite this
Kudou, M., Kubota, Y., Nakashima, N., Okazaki, F., Nakashima, K., Ogino, C., & Kondo, A. (2014). Improvement of enzymatic activity of β-glucosidase from Thermotoga maritima by 1-butyl-3-methylimidazolium acetate. Journal of Molecular Catalysis B: Enzymatic, 104, 17-22. https://doi.org/10.1016/j.molcatb.2014.02.013