Improvement of electrochemical conditions for detecting redox reaction of K3[Fe(CN)6] toward the application in norovirus aptasensor

Seiya Hirano, Junki Saito, Tomoki Yukawa, Daisuke Sano, Akihiro Okamoto, Satoshi Okabe, Masaaki Kitajima

Research output: Contribution to journalArticlepeer-review

Abstract

Electrochemical biosensors have attracted significant attention as a novel tool for the sensitive detection of pathogens and contaminants, with the potential capability of rapid, on-site diagnosis. In this study, we intended to improve the sensitivity of electrochemical biosensor using Fe(CN)6 3−/4 as redox marker, toward its application to norovirus aptasensors. Although many researchers have developed electrochemical aptasensors for various analytes using redox markers, the reported electrochemical conditions under which an aptasensor was examined varied across multiple studies. Here, we performed square-wave voltammetry (SWV) for electrodes modified with aptamer specific to murine norovirus and compared the reduction peak currents of Fe(CN)6 3− under various conditions. Effects of working electrode materials and NaCl and [Ru(NH3)6]Cl3 concentration in the electrolyte were examined. Among conditions we tested, the best sensitivity was obtained using a screen-printed gold electrode in an electrolyte containing 1 M NaCl with 4 mM K3[Fe(CN)6], in which the concentration of murine norovirus showed linearity with SWV peak current. This study provided useful information on the electrochemical measurement conditions regarding the development of electrochemical aptasensors.

Original languageEnglish
Pages (from-to)205-209
Number of pages5
JournalElectrochemistry
Volume88
Issue number3
DOIs
Publication statusPublished - 2020

Keywords

  • Aptamer
  • Biosensor
  • Norovirus
  • Screen-printed Electrode

ASJC Scopus subject areas

  • Electrochemistry

Fingerprint Dive into the research topics of 'Improvement of electrochemical conditions for detecting redox reaction of K<sub>3</sub>[Fe(CN)<sub>6</sub>] toward the application in norovirus aptasensor'. Together they form a unique fingerprint.

Cite this