Improved cellulose conversion to bio-hydrogen with thermophilic bacteria and characterization of microbial community in continuous bioreactor

Hongyu Jiang, Samir I. Gadow, Yasumitsu Tanaka, Jun Cheng, Yu You Li

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Thermophilic hydrogen fermentation of cellulose was evaluated by a long term continuous experiment and batch experiments. The continuous experiment was conducted under 55°C using a continuously stirred tank reactor (CSTR) at a hydraulic retention time (HRT) of 10 day. A stable hydrogen yield of 15.4±0.23molkg-1 of cellulose consumed was maintained for 190 days with acetate and butyrate as the main soluble byproducts. An analysis of the 16S rRNA sequences showed that the hydrogen-producing thermophilic cellulolytic microorganisms (HPTCM) were close to Thermoanaerobacterium thermosaccharolyticum, Clostridium sp. and Enterobacter cloacae. Batch experiment demonstrated that the highest H2 producing activity was obtained at 55°C and the ultimate hydrogen yield and the metabolic by-products were influenced greatly by temperatures. The effect of temperature variation showed that the activation energy for cellulose and glucose were estimated at 103 and 98.8kJmol-1, respectively.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalBiomass and Bioenergy
Volume75
DOIs
Publication statusPublished - 2015 Apr 1

Keywords

  • Activity test
  • Cellulose
  • Dark fermentation
  • Microbial community structure
  • Thermophilic

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal

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