Improved boronate affinity electrophoresis by optimization of the running buffer for a single-step separation of piRNA from mouse testis total RNA

Yusuke Sato, Daijiro Iwasawa, Kuo Ping Hui, Rena Nakagomi, Seiichi Nishizawa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Here we examined optimization of the running buffer in boronate affinity electrophoresis for improved separation of PIWI-interacting RNA (piRNA) with 2'-O-methylated ribose in 3'-terminal nucleotide. The use of Good's buffer, such as HEPES, significantly increased the separation efficiency for piRNA over normal RNA with free 3'-terminal ribose, and retained an ability to resolve the difference by at least 4-nucleotide lengths in the target piRNAs. We also demonstrated a single-step separation of piRNA from mouse testis total RNA.

Original languageEnglish
Pages (from-to)627-630
Number of pages4
Journalanalytical sciences
Volume34
Issue number5
DOIs
Publication statusPublished - 2018

Keywords

  • 2'-O-methylatyion
  • Boronate affinity electrophoresis
  • Phenylboronic acid
  • PiRNA
  • Separation

ASJC Scopus subject areas

  • Analytical Chemistry

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