Important role of heparan sulfate in postnatal islet growth and insulin secretion

Iwao Takahashi, Naoya Noguchi, Koji Nata, Shuhei Yamada, Tomoyuki Kaneiwa, Shuji Mizumoto, Takayuki Ikeda, Kazushi Sugihara, Masahide Asano, Takeo Yoshikawa, Akiyo Yamauchi, Nausheen Jamal Shervani, Akira Uruno, Ichiro Kato, Michiaki Unno, Kazuyuki Sugahara, Shin Takasawa, Hiroshi Okamoto, Akira Sugawara

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Heparan sulfate (HS) binds with several signaling molecules and regulates ligand-receptor interactions, playing an essential role in embryonic development. Here we showed that HS was intensively expressed in pancreatic islet β-cells after 1 week of age in mice. The enzymatic removal of HS in isolated islets resulted in attenuated glucose-induced insulin secretion with a concomitant reduction in gene expression of several key components in the insulin secretion machinery. We further depleted islet HS by inactivating the exostosin tumor-like 3 gene specifically in β-cells. These mice exhibited abnormal islet morphology with reduced β-cell proliferation after 1 week of age and glucose intolerance due to defective insulin secretion. These results demonstrate that islet HS is involved in the regulation of postnatal islet maturation and required to ensure normal insulin secretion.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalBiochemical and biophysical research communications
Volume383
Issue number1
DOIs
Publication statusPublished - 2009 May 22

Keywords

  • Extl3
  • Heparan sulfate
  • Insulin secretion
  • β-Cell proliferation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Important role of heparan sulfate in postnatal islet growth and insulin secretion'. Together they form a unique fingerprint.

Cite this