The role of glutathione (GSH) as a determinant of susceptibility to methylmercury (MeHg) in mammalian cells was studied by using PC12 cells, seven sublines with different levels of intracellular GSH, and seven cell lines derived from different organs or different species of animals. The PC12/TM cell line contains four times higher GSH level than the parent PC12 cells. The pretreatment with buthionine sulfoximine (BSO), which reduced the GSH level to that of parent cells, increased the sensitivity of the cells to MeHg. The intracellular GSH level was significantly correlated with the sensitivity to MeHg in PC12 and its seven sublines. Furthermore, the same correlation as above was observed among various cell lines derived from different organs or different species of animals. These results show that the level of cellular GSH is a determinant of cell susceptibility to MeHg, and that the neuronal cell lines examined show a tendency to contain lower GSH level and consequently, a higher susceptibility to MeHg than nonneuronal cell lines.
|Number of pages||6|
|Journal||In Vitro Toxicology: Journal of Molecular and Cellular Toxicology|
|Publication status||Published - 1994 Jan 1|
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