Importance of cell damage causing growth delay for high pressure inactivation of Saccharomyces cerevisiae

Masaru Nanba, Kazuki Nomura, Yusuke Nasuhara, Manabu Hayashi, Miyuki Kido, Mayumi Hayashi, Akinori Iguchi, Toru Shigematsu, Masao Hirayama, Shigeaki Ueno, Tomoyuki Fujii

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A high pressure (HP) tolerant (barotolerant) mutant a2568D8 and a variably barotolerant mutant a1210H12 were generated from Saccharomyces cerevisiae using ultra-violet mutagenesis. The two mutants, a barosensitive mutant a924E1 and the wild-type strain, were pressurized (225 MPa), and pressure inactivation behavior was analyzed. In the wild-type strain, a proportion of the growth-delayed cells were detected after exposure to HP. In a924E1, the proportion of growth-delayed cells significantly decreased compared with the wild-type. In a2568D8, the proportion of growth-delayed cells increased and the proportion of inactivated cells decreased compared with the wild-type. In a1210H12, the growth-delayed cells could not be detected within 120 s of exposure to HP. The proportion of growth-delayed cells, which incurred the damage, would affect the survival ratio by HP. These results suggested that cellular changes in barotolerance caused by mutations are remarkably affected by the ability to recover from cellular damage, which results in a growth delay.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalHigh Pressure Research
Volume33
Issue number2
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • barosensitivity
  • barotolerance
  • high hydrostatic pressure
  • ultra-violet mutagenesis
  • yeast

ASJC Scopus subject areas

  • Condensed Matter Physics

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