TY - JOUR
T1 - Importance of cell damage causing growth delay for high pressure inactivation of Saccharomyces cerevisiae
AU - Nanba, Masaru
AU - Nomura, Kazuki
AU - Nasuhara, Yusuke
AU - Hayashi, Manabu
AU - Kido, Miyuki
AU - Hayashi, Mayumi
AU - Iguchi, Akinori
AU - Shigematsu, Toru
AU - Hirayama, Masao
AU - Ueno, Shigeaki
AU - Fujii, Tomoyuki
PY - 2013/6/1
Y1 - 2013/6/1
N2 - A high pressure (HP) tolerant (barotolerant) mutant a2568D8 and a variably barotolerant mutant a1210H12 were generated from Saccharomyces cerevisiae using ultra-violet mutagenesis. The two mutants, a barosensitive mutant a924E1 and the wild-type strain, were pressurized (225 MPa), and pressure inactivation behavior was analyzed. In the wild-type strain, a proportion of the growth-delayed cells were detected after exposure to HP. In a924E1, the proportion of growth-delayed cells significantly decreased compared with the wild-type. In a2568D8, the proportion of growth-delayed cells increased and the proportion of inactivated cells decreased compared with the wild-type. In a1210H12, the growth-delayed cells could not be detected within 120 s of exposure to HP. The proportion of growth-delayed cells, which incurred the damage, would affect the survival ratio by HP. These results suggested that cellular changes in barotolerance caused by mutations are remarkably affected by the ability to recover from cellular damage, which results in a growth delay.
AB - A high pressure (HP) tolerant (barotolerant) mutant a2568D8 and a variably barotolerant mutant a1210H12 were generated from Saccharomyces cerevisiae using ultra-violet mutagenesis. The two mutants, a barosensitive mutant a924E1 and the wild-type strain, were pressurized (225 MPa), and pressure inactivation behavior was analyzed. In the wild-type strain, a proportion of the growth-delayed cells were detected after exposure to HP. In a924E1, the proportion of growth-delayed cells significantly decreased compared with the wild-type. In a2568D8, the proportion of growth-delayed cells increased and the proportion of inactivated cells decreased compared with the wild-type. In a1210H12, the growth-delayed cells could not be detected within 120 s of exposure to HP. The proportion of growth-delayed cells, which incurred the damage, would affect the survival ratio by HP. These results suggested that cellular changes in barotolerance caused by mutations are remarkably affected by the ability to recover from cellular damage, which results in a growth delay.
KW - barosensitivity
KW - barotolerance
KW - high hydrostatic pressure
KW - ultra-violet mutagenesis
KW - yeast
UR - http://www.scopus.com/inward/record.url?scp=84881220202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881220202&partnerID=8YFLogxK
U2 - 10.1080/08957959.2013.781596
DO - 10.1080/08957959.2013.781596
M3 - Article
AN - SCOPUS:84881220202
VL - 33
SP - 299
EP - 307
JO - High Pressure Research
JF - High Pressure Research
SN - 0895-7959
IS - 2
ER -