Implications of coordinated cell-body rotations for Leptospira motility

Kyosuke Takabe, Akihiro Kawamoto, Hajime Tahara, Seishi Kudo, Shuichi Nakamura

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The spirochete Leptospira has a coiled cell body and two periplasmic flagella (PFs) that reside beneath the outer sheath. PFs extend from each end of the cell body and are attached to the right-handed spiral protoplasmic cylinder (PC) via a connection with the flagellar motor embedded in the inner membrane. PFs bend each end of the cell body into left-handed spiral (S) or planar hook (H) shapes, allowing leptospiral cells to swim using combined anterior S-end and posterior H-end gyrations with PC rotations. As a plausible mechanism for motility, S- and H-end gyrations by PFs and PC rotations by PF countertorque imply mutual influences among the three parts. Here we show a correlation between H-end gyration and PC rotation from the time records of rotation rates and rotational directions of individual swimming cells. We then qualitatively explain the observed correlation using a simple rotation model based on the measurements of motility and intracellular arrangements of PFs revealed by cryo-electron microscopy and electron cryotomography.

Original languageEnglish
Pages (from-to)1040-1046
Number of pages7
JournalBiochemical and biophysical research communications
Volume491
Issue number4
DOIs
Publication statusPublished - 2017 Sep 30

Keywords

  • Bacterial motility
  • Cross-correlation
  • Leptospira
  • Spirochete

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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