Impairment of transalveolar fluid transport and lung Na+-K+-ATPase function by hypoxia in rats

Satoshi Suzuki, Masafumi Noda, Makoto Sugita, Sadafumi Ono, Kaoru Koike, Shigefumi Fujimura

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55 Citations (Scopus)

Abstract

We examined whether hypoxic exposure in vivo would influence transalveolar fluid transport in rats. We found a significant decrease in alveolar fluid clearance of the rats exposed to 10% oxygen for 48 h. Terbutaline did not stimulate alveolar fluid clearance, and alveolar fluid cAMP levels were lower than those determined in normoxia experiment. Hypoxia did not influence the alveolar fluid lactate dehydrogenase levels, Evans blue dye fluid-to-serum concentration ratio, or lung wet-to-dry weight ratio, indicating no significant change in the permeability of alveolar-capillary barrier. Histological examination showed no significant fluid accumulation into the interstitium and the alveolar space. Hypoxia did not reduce lung ATP content; however, we found significant decrease in Na+-K+-ATPase hydrolytic activity in lung tissue preparations and isolated alveolar type II cells. Our data indicate that hypoxic exposure in vivo impairs transalveolar fluid transport, and this impairment is related to the decrease in alveolar epithelial Na+-K+-ATPase hydrolytic activity but is not secondary to the alteration of cellular energy source.

Original languageEnglish
Pages (from-to)962-968
Number of pages7
JournalJournal of Applied Physiology
Volume87
Issue number3
DOIs
Publication statusPublished - 1999 Sep

Keywords

  • Alveolar fluid clearance
  • Alveolar type II cells
  • Hypoxia
  • β-adrenergic agonists

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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