Impaired colonization of the gonads by primordial germ cells in mice lacking a chemokine, stromal cell-derived factor-1 (SDF-1)

Toshiaki Ara, Yuri Nakamura, Takeshi Egawa, Tatsuki Sugiyama, Kuniya Abe, Tadamitsu Kishimoto, Yasuhisa Matsui, Takashi Nagasawa

Research output: Contribution to journalArticlepeer-review

247 Citations (Scopus)

Abstract

Primordial germ cells (PGCs) are the founders of sperm or oocytes. PGCs migrate through the tissues of the embryos and colonize the gonads during development. However, the cytokines essential for colonization of the gonads by PGCs in mammals remain unclear. Stromal cell-derived factor-1 (SDF-1, also called PBSF and CXCL12) is a member of chemokines, a family of structurally related chemoattractive cytokines. SDF-1 and its primary physiologic receptor CXCR4 have multiple essential functions in development including colonization of bone marrow by hematopoietic cells and neuron localization within cerebellum during embryogenesis as well as B lymphopoiesis and cardiovasculogenesis. Here, we have shown that PGCs have cell-surface expression of CXCR4 and that, in SDF-1-/- mice, PGCs undergo directed migration through tissues of embryos, but the numbers of PGCs in the gonads are significantly reduced. The proliferation of PGCs within the gonads seems normal in the mutant mice. These findings reveal the essential role for SDF-1 in murine PGC development likely by controlling colonization of the gonads by PGCs.

Original languageEnglish
Pages (from-to)5319-5323
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number9
DOIs
Publication statusPublished - 2003 Apr 29
Externally publishedYes

ASJC Scopus subject areas

  • General

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