Impact of Tissue Sealing Sheet on Erectile Dysfunction in a Rat Model of Nerve-Sparing Radical Prostatectomy

Shinichi Yamashita, Shinji Fujii, Yoshihiro Kamiyama, Yoshihide Kawasaki, Hideaki Izumi, Naoki Kawamorita, Koji Mitsuzuka, Hisanobu Adachi, Yasuhiro Kaiho, Akihiro Ito, Yoichi Arai

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Introduction The tissue sealing sheet has recently been used to prevent intraoperative bleeding from the neurovascular bundles in radical prostatectomy. Surgical stress or inflammatory changes likely play a role in erectile dysfunction after cavernous nerve injury. However, the efficacy of a tissue sealing sheet for preventing erectile function after nerve-sparing radical prostatectomy remains unclear. Aim To evaluate the effect of a tissue sealing sheet on erectile dysfunction after cavernous nerve dissection. Methods Male Sprague-Dawley rats were randomly divided into three groups and subjected to sham operation or bilateral cavernous nerve dissection with (sheet group) or without (non-sheet group) a tissue sealing sheet. In the sheet group, cavernous nerves were sealed with a tissue sealing sheet immediately after cavernous nerve dissection. Main Outcome Measures Erectile function was assessed by measuring intracavernous pressure and arterial pressure during pelvic nerve electrostimulation at 4 weeks after surgery. Expressions of interleukin-6, tumor growth factor-β1, and heme-oxygenase-1 in the major pelvic ganglion were examined by real-time polymerase chain reaction. Results Mean intracavernous pressure along with mean arterial pressure in the sheet group were similar to those in the sham group and showed a significant positive response compared with the non-sheet group (P < .05). Furthermore, expressions of interleukin-6, tumor growth factor-β1, and heme-oxygenase-1 were significantly lower in the sheet group than in the non-sheet group (P < .05). Conclusion Use of a tissue sealing sheet attenuated postoperative inflammatory changes and oxidative stress and improved erectile function after cavernous nerve injury in rats. The tissue sealing sheet might become a useful therapeutic approach to preserve erectile function after nerve-sparing radical prostatectomy.

Original languageEnglish
Pages (from-to)1448-1454
Number of pages7
JournalJournal of Sexual Medicine
Volume13
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Cavernous Nerve Injury
  • Erectile Dysfunction
  • Nerve-Sparing Radical Prostatectomy
  • Tissue Sealing Sheet

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Urology

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