Impact of fasting time on hepatic lipid metabolism in nutritional animal studies

Ikuo Ikeda, Kanae Metoki, Takashi Yamahira, Masaki Kato, Nao Inoue, Koji Nagao, Teruyoshi Yanagita, Hitoshi Shirakawa, Michio Komai

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Many animal studies on improvement of lipid metabolism, using dietary components, fast the animals on the final day of the feeding. Although fasting has a significant impact on lipid metabolism, its time-dependent influence is not fully understood. We examined the effects of several fasting times on lipid metabolism. Rats fed with a semisynthetic diet for 2 wk were killed after 0 (9:00 am), 6 (7:00 am-1:00 pm), 9 (0:00 am-9:00 am), and 13 h (8:00 pm-9:00 am) of fasting. Co mpared to the 0 h group, marked reduction of liver weight and hepatic triacylglycerol content was observed in the 9 and 13 h groups. Activities of hepatic enzymes involved in fatty acid synthesis gradually decreased during fasting. In contrast, drastic time-dependent reduction of gene expression, of the enzymes, was observed. Expression of carnitine palmitoyltransfer-ase mRNA was higher in the fasting groups than in the 0 h group. Our study showed that fasting has a significant impact on several parameters related to lipid metabolism in rat liver.

Original languageEnglish
Pages (from-to)1584-1591
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Issue number9
Publication statusPublished - 2014


  • Abdominal fat
  • Fatty acid synthesis
  • Liver
  • Triacylglycerol
  • β-oxidation

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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