Immunostimulatory activity of polysaccharides isolated from caulerpa lentillifera on macrophage cells

Reiko Maeda, Tomoaki Ida, Hideshi Ihara, Tatsuji Sakamoto

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)


Polysaccharides were extracted from Caulerpa lentillifera by treating with water and then purified by size-exclusion chromatography. The purified polysaccharides, termed SP1, were found to be sulfated xylogalactans with a molecular mass of more than 100 kDa. Adding SP1 to murine macrophage RAW 264.7 cells increased the production of nitric oxide (NO) in a dose-dependent manner. NO was found by immunoblotting and RT-PCR analyses to be synthesized by an inducible NO synthase. SP1 caused the degradation of IκB-α and the nuclear translocation of nuclear factor (NF)-κB subunit p65 in macrophage cells. SP1 also increased the phosphorylation of p38 mitogen-activated protein kinase (MAPK). These results demonstrate that SP1 activated macrophage cells via both the NF-κB and p38 MAPK signaling pathways. Moreover, SP1 increased the expression of various genes encoding cytokines, and the phagocytic activity of macrophage cells. These combined results show that SP1 immunostimulated the activity of macrophage cells.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2012
Externally publishedYes


  • Caulerpa lentillifera
  • Cytokine
  • Immunostimulatory activity
  • Nitric oxide
  • Phagocytosis

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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