Immunostaining of stage-specific embryonic antigen-4 in intratubular germ cell neoplasia unclassified and in testicular germ-cell tumors

Satoru Tokuyama, Seiichi Saito, Toshiko Takahashi, Chikara Ohyama, I. T.O. Akihiro, Satoru Kanto, Makoto Satoh, Senji Hoshi, Mareyuki Endoh, Yoichi Arai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Stage-specific embryonic antigen-4 (SSEA-4) is expressed in testicular germ-cell tumors (GCT) according to studies using thin-layer chromatography (TLC) immunostaining. To further understand the relationship between SSEA-4 and the histogenesis of testicular GCT, we examined the expression of SSEA-4 in 43 samples of testicular GCT either by immunohistochemical staining or by TLC immunostaining. Immunohistochemical staining detected SSEA-4 in spermatogonia from the non-tumor parts of the testis and in all of 8 samples of intratubular germ cell neoplasia unclassified (IGCNU). Immunohistochemical staining was SSEA-4 positive in all 9 seminomas, and in one yolk sac tumor and in 5 embryonal carcinomas among 9 non-seminomas. Immunostaining with TLC showed that SSEA-4 was retained in 15 of 16 seminomas and in 4 of 8 non-seminomas. These results demonstrate an antigenic link between spermatogonia, IGCNU, and testicular GCT. We suggest that SSEA-4 is associated with the histogenesis of testicular GCT.

Original languageEnglish
Pages (from-to)1097-1104
Number of pages8
JournalOncology reports
Volume10
Issue number5
DOIs
Publication statusPublished - 2003 Sep

Keywords

  • Globo-series glycolipids
  • Intratubular germ cell neoplasia unclassified
  • Spermatogonia
  • Stage-specific embryonic antigen-4
  • Testicular germ-cell tumor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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